Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
117 | 3' | -59.4 | AC_000006.1 | + | 21893 | 0.66 | 0.353072 |
Target: 5'- -cUGCUGCGUGCaggauGCC-GGGUGCgGGAa -3' miRNA: 3'- ucACGGUGUACG-----UGGaCCCGCGgUCU- -5' |
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117 | 3' | -59.4 | AC_000006.1 | + | 21693 | 0.66 | 0.353072 |
Target: 5'- cGUGCCAUu----CCUGGGuCGCCAa- -3' miRNA: 3'- uCACGGUGuacguGGACCC-GCGGUcu -5' |
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117 | 3' | -59.4 | AC_000006.1 | + | 21320 | 0.66 | 0.336161 |
Target: 5'- gAGgGCCACGUugcgguacugGUACUUGGGCuGCCAc- -3' miRNA: 3'- -UCaCGGUGUA----------CGUGGACCCG-CGGUcu -5' |
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117 | 3' | -59.4 | AC_000006.1 | + | 15438 | 0.66 | 0.327114 |
Target: 5'- cGUGCUcCGaaugGCGCCUGGGCgauccguccgcccGCCGGc -3' miRNA: 3'- uCACGGuGUa---CGUGGACCCG-------------CGGUCu -5' |
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117 | 3' | -59.4 | AC_000006.1 | + | 16561 | 0.67 | 0.289022 |
Target: 5'- gGGUGCCGCGguaGCCc-GGCGUCGGGa -3' miRNA: 3'- -UCACGGUGUacgUGGacCCGCGGUCU- -5' |
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117 | 3' | -59.4 | AC_000006.1 | + | 8800 | 0.67 | 0.277362 |
Target: 5'- cGUGCCGCGcgaagacggcguaguUGCGCa--GGCGCUGGAa -3' miRNA: 3'- uCACGGUGU---------------ACGUGgacCCGCGGUCU- -5' |
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117 | 3' | -59.4 | AC_000006.1 | + | 16270 | 0.7 | 0.194061 |
Target: 5'- aGGUGgCGCcgG-GCCUGGGCGUgCAGAc -3' miRNA: 3'- -UCACgGUGuaCgUGGACCCGCG-GUCU- -5' |
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117 | 3' | -59.4 | AC_000006.1 | + | 8771 | 0.7 | 0.194061 |
Target: 5'- --aGCCGCGUcuggaacgagaGCgGCCUGGGCGCCu-- -3' miRNA: 3'- ucaCGGUGUA-----------CG-UGGACCCGCGGucu -5' |
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117 | 3' | -59.4 | AC_000006.1 | + | 3039 | 1.08 | 0.000186 |
Target: 5'- aAGUGCCACAUGCACCUGGGCGCCAGAa -3' miRNA: 3'- -UCACGGUGUACGUGGACCCGCGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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