Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11703 | 3' | -52.6 | NC_003102.1 | + | 111965 | 0.66 | 0.966997 |
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Target: 5'- --aUCGGUgUCGACuccUGcGCCGCCUc -3' miRNA: 3'- acaAGCCGgAGUUGua-ACuCGGUGGG- -5' |
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| 11703 | 3' | -52.6 | NC_003102.1 | + | 5765 | 0.66 | 0.966018 |
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Target: 5'- aGUUCGGCCUUuuAUAgucGGGCaggaucgauuguuaCGCCCg -3' miRNA: 3'- aCAAGCCGGAGu-UGUaa-CUCG--------------GUGGG- -5' |
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| 11703 | 3' | -52.6 | NC_003102.1 | + | 49272 | 0.66 | 0.963653 |
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Target: 5'- cGUUC-GCgUC-GCGUcuGGCCACCCa -3' miRNA: 3'- aCAAGcCGgAGuUGUAacUCGGUGGG- -5' |
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| 11703 | 3' | -52.6 | NC_003102.1 | + | 35046 | 0.66 | 0.963653 |
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Target: 5'- --gUC-GCCgUCGACGaUGAGaCCACCCc -3' miRNA: 3'- acaAGcCGG-AGUUGUaACUC-GGUGGG- -5' |
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| 11703 | 3' | -52.6 | NC_003102.1 | + | 119428 | 0.67 | 0.956271 |
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Target: 5'- -uUUUGGCuCUCAACAaaucgGGGCgCACCa -3' miRNA: 3'- acAAGCCG-GAGUUGUaa---CUCG-GUGGg -5' |
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| 11703 | 3' | -52.6 | NC_003102.1 | + | 42318 | 0.67 | 0.947929 |
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Target: 5'- --aUCGGCCcacUCGAUcacGGGCaCACCCg -3' miRNA: 3'- acaAGCCGG---AGUUGuaaCUCG-GUGGG- -5' |
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| 11703 | 3' | -52.6 | NC_003102.1 | + | 19969 | 0.68 | 0.933553 |
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Target: 5'- ---aUGGCgCUCGACGcgaGAGCCgcGCCCa -3' miRNA: 3'- acaaGCCG-GAGUUGUaa-CUCGG--UGGG- -5' |
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| 11703 | 3' | -52.6 | NC_003102.1 | + | 81482 | 0.68 | 0.919249 |
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Target: 5'- cGUaCGGUugcccagaucgaucaCUCGGCGUagcUGAGCUGCCCc -3' miRNA: 3'- aCAaGCCG---------------GAGUUGUA---ACUCGGUGGG- -5' |
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| 11703 | 3' | -52.6 | NC_003102.1 | + | 86888 | 0.76 | 0.535549 |
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Target: 5'- ---gUGGCCUCGcaACAUcgUGGGCCACCUu -3' miRNA: 3'- acaaGCCGGAGU--UGUA--ACUCGGUGGG- -5' |
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| 11703 | 3' | -52.6 | NC_003102.1 | + | 120737 | 1 | 0.021596 |
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Target: 5'- cUGUUCGGCCUCAACAUUGAGC-ACCCg -3' miRNA: 3'- -ACAAGCCGGAGUUGUAACUCGgUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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