Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11704 | 5' | -49.6 | NC_003102.1 | + | 17499 | 0.71 | 0.945055 |
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Target: 5'- aCGGAUCCGAUguacagaucgcgUCGGCGGGuauGUCgAUGg -3' miRNA: 3'- -GCCUAGGCUA------------AGCUGCUU---UAGgUGCa -5' |
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| 11704 | 5' | -49.6 | NC_003102.1 | + | 18492 | 0.67 | 0.99577 |
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Target: 5'- gGGcgCCG--UCGACGAuGUUCGCGc -3' miRNA: 3'- gCCuaGGCuaAGCUGCUuUAGGUGCa -5' |
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| 11704 | 5' | -49.6 | NC_003102.1 | + | 20165 | 0.68 | 0.988532 |
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Target: 5'- cCGuGUCCGAuUUUGACGAGAUCaCGCu- -3' miRNA: 3'- -GCcUAGGCU-AAGCUGCUUUAG-GUGca -5' |
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| 11704 | 5' | -49.6 | NC_003102.1 | + | 47367 | 0.71 | 0.949519 |
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Target: 5'- gGGAacgCCGAUUCGAUGAcGGUCCAa-- -3' miRNA: 3'- gCCUa--GGCUAAGCUGCU-UUAGGUgca -5' |
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| 11704 | 5' | -49.6 | NC_003102.1 | + | 70324 | 0.68 | 0.992335 |
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Target: 5'- aCGGAUCauGUUCGAuCGAGguuuGUCCACu- -3' miRNA: 3'- -GCCUAGgcUAAGCU-GCUU----UAGGUGca -5' |
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| 11704 | 5' | -49.6 | NC_003102.1 | + | 72129 | 0.66 | 0.99849 |
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Target: 5'- gGGAUCgGGguguUUCuGGCGAucgcGUCCGCGa -3' miRNA: 3'- gCCUAGgCU----AAG-CUGCUu---UAGGUGCa -5' |
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| 11704 | 5' | -49.6 | NC_003102.1 | + | 120593 | 0.67 | 0.99577 |
|
Target: 5'- uCGGGUCgGAcUUCGuCGGGAagCACGUu -3' miRNA: 3'- -GCCUAGgCU-AAGCuGCUUUagGUGCA- -5' |
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| 11704 | 5' | -49.6 | NC_003102.1 | + | 121009 | 0.66 | 0.99849 |
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Target: 5'- -cGAUCCGAUcacgGGCGAGcgCCACa- -3' miRNA: 3'- gcCUAGGCUAag--CUGCUUuaGGUGca -5' |
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| 11704 | 5' | -49.6 | NC_003102.1 | + | 127213 | 1.08 | 0.015721 |
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Target: 5'- gCGGAUCCGAUUCGACGAAAUCCACGUc -3' miRNA: 3'- -GCCUAGGCUAAGCUGCUUUAGGUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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