Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11705 | 5' | -52.4 | NC_003102.1 | + | 136834 | 1.1 | 0.005407 |
Target: 5'- uCGAUCAUGUUUUCGGCCCACCAGCGGc -3' miRNA: 3'- -GCUAGUACAAAAGCCGGGUGGUCGCC- -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 134392 | 0.74 | 0.718719 |
Target: 5'- gCGGUCGcaagaaUUUGGCgCCGCCGGCGGa -3' miRNA: 3'- -GCUAGUacaa--AAGCCG-GGUGGUCGCC- -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 129769 | 0.67 | 0.9643 |
Target: 5'- aCGAUagcaacuUGaugUCGGCCgACCGGCGa -3' miRNA: 3'- -GCUAgu-----ACaaaAGCCGGgUGGUCGCc -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 114978 | 0.68 | 0.941715 |
Target: 5'- aCGAUCGUGUcUauagacucgaucgaaUCGGUCaCAUUGGCGGu -3' miRNA: 3'- -GCUAGUACAaA---------------AGCCGG-GUGGUCGCC- -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 85023 | 0.69 | 0.918601 |
Target: 5'- aCGGUCAcGUUg--GGCCaguacacguacgCGCCGGCGGg -3' miRNA: 3'- -GCUAGUaCAAaagCCGG------------GUGGUCGCC- -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 80515 | 0.66 | 0.984749 |
Target: 5'- -aAUCAUGUUUUCGGaauCACCGuuGCGa -3' miRNA: 3'- gcUAGUACAAAAGCCgg-GUGGU--CGCc -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 70776 | 0.67 | 0.957085 |
Target: 5'- gCGAUCGUGUUUuguaucaugcUCGGC--ACCGGCa- -3' miRNA: 3'- -GCUAGUACAAA----------AGCCGggUGGUCGcc -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 31881 | 0.66 | 0.973461 |
Target: 5'- aCGAUCGg----UCGcGCCCGCCgAGCa- -3' miRNA: 3'- -GCUAGUacaaaAGC-CGGGUGG-UCGcc -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 17299 | 0.68 | 0.939812 |
Target: 5'- cCGAUCGUGcgg-CGuGCCCGCCAuCGa -3' miRNA: 3'- -GCUAGUACaaaaGC-CGGGUGGUcGCc -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 15103 | 0.66 | 0.973461 |
Target: 5'- uGAUCAUcaUUcgaUCGGUCgcgaACCGGCGGu -3' miRNA: 3'- gCUAGUAcaAA---AGCCGGg---UGGUCGCC- -5' |
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11705 | 5' | -52.4 | NC_003102.1 | + | 2940 | 0.66 | 0.980784 |
Target: 5'- aCGAgacCAUGgacUUUUGGgCCAUCGGCGu -3' miRNA: 3'- -GCUa--GUACa--AAAGCCgGGUGGUCGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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