Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1171 | 5' | -50.8 | NC_001132.2 | + | 150250 | 1.11 | 0.008453 |
Target: 5'- uCCCACGAGUCGUUCCUUCAACCGAUCc -3' miRNA: 3'- -GGGUGCUCAGCAAGGAAGUUGGCUAG- -5' |
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1171 | 5' | -50.8 | NC_001132.2 | + | 137524 | 0.7 | 0.9611 |
Target: 5'- aCCACGGGUCGUUCgUaaAGCCuGAg- -3' miRNA: 3'- gGGUGCUCAGCAAGgAagUUGG-CUag -5' |
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1171 | 5' | -50.8 | NC_001132.2 | + | 92295 | 0.7 | 0.957409 |
Target: 5'- cUCCACGAGUUGcgUCCguaacgUCGACgCGAg- -3' miRNA: 3'- -GGGUGCUCAGCa-AGGa-----AGUUG-GCUag -5' |
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1171 | 5' | -50.8 | NC_001132.2 | + | 73778 | 0.68 | 0.984842 |
Target: 5'- uCCUcgACGuAGUCcuucUCCUUgAACCGAUCg -3' miRNA: 3'- -GGG--UGC-UCAGca--AGGAAgUUGGCUAG- -5' |
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1171 | 5' | -50.8 | NC_001132.2 | + | 3717 | 0.67 | 0.990457 |
Target: 5'- uCCCAagaaggacgacgacCGGGUCGUugUCC--CGGCCGAUUg -3' miRNA: 3'- -GGGU--------------GCUCAGCA--AGGaaGUUGGCUAG- -5' |
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1171 | 5' | -50.8 | NC_001132.2 | + | 60782 | 0.66 | 0.994775 |
Target: 5'- aCCCuagACGgauacAGUUGUUUCUUCAACUG-UCg -3' miRNA: 3'- -GGG---UGC-----UCAGCAAGGAAGUUGGCuAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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