miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1171 5' -50.8 NC_001132.2 + 150250 1.11 0.008453
Target:  5'- uCCCACGAGUCGUUCCUUCAACCGAUCc -3'
miRNA:   3'- -GGGUGCUCAGCAAGGAAGUUGGCUAG- -5'
1171 5' -50.8 NC_001132.2 + 137524 0.7 0.9611
Target:  5'- aCCACGGGUCGUUCgUaaAGCCuGAg- -3'
miRNA:   3'- gGGUGCUCAGCAAGgAagUUGG-CUag -5'
1171 5' -50.8 NC_001132.2 + 92295 0.7 0.957409
Target:  5'- cUCCACGAGUUGcgUCCguaacgUCGACgCGAg- -3'
miRNA:   3'- -GGGUGCUCAGCa-AGGa-----AGUUG-GCUag -5'
1171 5' -50.8 NC_001132.2 + 73778 0.68 0.984842
Target:  5'- uCCUcgACGuAGUCcuucUCCUUgAACCGAUCg -3'
miRNA:   3'- -GGG--UGC-UCAGca--AGGAAgUUGGCUAG- -5'
1171 5' -50.8 NC_001132.2 + 60782 0.66 0.994775
Target:  5'- aCCCuagACGgauacAGUUGUUUCUUCAACUG-UCg -3'
miRNA:   3'- -GGG---UGC-----UCAGCAAGGAAGUUGGCuAG- -5'
1171 5' -50.8 NC_001132.2 + 3717 0.67 0.990457
Target:  5'- uCCCAagaaggacgacgacCGGGUCGUugUCC--CGGCCGAUUg -3'
miRNA:   3'- -GGGU--------------GCUCAGCA--AGGaaGUUGGCUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.