miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11720 5' -46.8 NC_003113.1 + 1417 0.7 0.44664
Target:  5'- gGUUACAGCU-CAGGgugaCGAGGAUAc- -3'
miRNA:   3'- aCAAUGUUGAuGUCCg---GCUCUUAUca -5'
11720 5' -46.8 NC_003113.1 + 3144 0.72 0.34655
Target:  5'- -aUUGCGACUACAgcgacGGCUGAGGccucuGUAGUg -3'
miRNA:   3'- acAAUGUUGAUGU-----CCGGCUCU-----UAUCA- -5'
11720 5' -46.8 NC_003113.1 + 2473 1.1 0.000625
Target:  5'- uUGUUACAACUACAGGCCGAGAAUAGUg -3'
miRNA:   3'- -ACAAUGUUGAUGUCCGGCUCUUAUCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.