miRNA: 3'- gUC-UGCGGCCGCAuGA----AAGCUG-CGcc -5'
miRNA: 3'- -GUCUGCGGCCGCAUGAAAGCUGCGCC- -5'
Results 1 - 2 of 2 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11726 | 3' | -55.1 | NC_003199.1 | + | 338 | 0.66 | 0.07653 |
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Target: 5'- aAGuACGCCGGCGU-CUgaacUUCGGCaGCc- -3' miRNA: 3'- gUC-UGCGGCCGCAuGA----AAGCUG-CGcc -5' |
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| 11726 | 3' | -55.1 | NC_003199.1 | + | 357 | 1.11 | 0.000008 |
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Target: 5'- uCAGACGCCGGCGUACUUUCGACGCGGg -3' miRNA: 3'- -GUCUGCGGCCGCAUGAAAGCUGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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