Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11733 | 3' | -50.5 | NC_003215.1 | + | 7853 | 0.66 | 0.483462 |
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Target: 5'- gCAGGUUUcaugacuuugUCGCCCAccagGCAgGCGUa- -3' miRNA: 3'- gGUCCGAAa---------AGCGGGU----UGUgUGCAag -5' |
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| 11733 | 3' | -50.5 | NC_003215.1 | + | 3621 | 0.67 | 0.471659 |
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Target: 5'- aCCuGGCU--UUGCCuCGACGCAgGgUCa -3' miRNA: 3'- -GGuCCGAaaAGCGG-GUUGUGUgCaAG- -5' |
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| 11733 | 3' | -50.5 | NC_003215.1 | + | 4815 | 0.67 | 0.42599 |
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Target: 5'- uCCAuug-UUUCGCCCAGCGCGuauaggcauaucCGUUCg -3' miRNA: 3'- -GGUccgaAAAGCGGGUUGUGU------------GCAAG- -5' |
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| 11733 | 3' | -50.5 | NC_003215.1 | + | 3082 | 0.68 | 0.383056 |
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Target: 5'- aCC-GGCUg--CGCCUGugGaCGCGUUCc -3' miRNA: 3'- -GGuCCGAaaaGCGGGUugU-GUGCAAG- -5' |
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| 11733 | 3' | -50.5 | NC_003215.1 | + | 7047 | 0.77 | 0.102517 |
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Target: 5'- -gGGGUUUUUCGCCCAugACAgCGUc- -3' miRNA: 3'- ggUCCGAAAAGCGGGUugUGU-GCAag -5' |
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| 11733 | 3' | -50.5 | NC_003215.1 | + | 3155 | 1.14 | 0.000161 |
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Target: 5'- aCCAGGCUUUUCGCCCAACACACGUUCg -3' miRNA: 3'- -GGUCCGAAAAGCGGGUUGUGUGCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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