miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11734 3' -55.8 NC_003215.1 + 7492 0.65 0.292117
Target:  5'- gCCAAGgaCGGGCC-GCCGgGCGcgggcgccaccggCGGCCg -3'
miRNA:   3'- -GGUUCa-GCUUGGaCGGC-CGCa------------GUCGG- -5'
11734 3' -55.8 NC_003215.1 + 1513 0.67 0.235445
Target:  5'- aCCAAG-CGAgaguuaauACCUGUucucgacgCGuCGUCAGCCa -3'
miRNA:   3'- -GGUUCaGCU--------UGGACG--------GCcGCAGUCGG- -5'
11734 3' -55.8 NC_003215.1 + 5271 0.76 0.051432
Target:  5'- gCCAGGUCcgcgauGCCUGCgGGUucaggguguacGUCAGCCg -3'
miRNA:   3'- -GGUUCAGcu----UGGACGgCCG-----------CAGUCGG- -5'
11734 3' -55.8 NC_003215.1 + 3748 1.15 0.000038
Target:  5'- aCCAAGUCGAACCUGCCGGCGUCAGCCg -3'
miRNA:   3'- -GGUUCAGCUUGGACGGCCGCAGUCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.