Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11736 | 3' | -46.3 | NC_003215.1 | + | 8102 | 0.66 | 0.778067 |
Target: 5'- uUGUUGUCAAAGAUg--GGccggccacuGUCUCCCg -3' miRNA: 3'- uGCGACAGUUUUUGauaCU---------UAGGGGG- -5' |
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11736 | 3' | -46.3 | NC_003215.1 | + | 10347 | 0.67 | 0.741156 |
Target: 5'- cCGCUGUCAucGGCc-UGGA-CCCCg -3' miRNA: 3'- uGCGACAGUuuUUGauACUUaGGGGg -5' |
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11736 | 3' | -46.3 | NC_003215.1 | + | 9357 | 0.67 | 0.715721 |
Target: 5'- cCGCUGUCAcccauuccucguGAuACUGcGggUCCUCa -3' miRNA: 3'- uGCGACAGU------------UUuUGAUaCuuAGGGGg -5' |
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11736 | 3' | -46.3 | NC_003215.1 | + | 10722 | 0.68 | 0.689811 |
Target: 5'- gGCGCUGUCAGgcAAAUUcAUGGagacagggauGUUUCCCa -3' miRNA: 3'- -UGCGACAGUU--UUUGA-UACU----------UAGGGGG- -5' |
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11736 | 3' | -46.3 | NC_003215.1 | + | 7012 | 0.73 | 0.366543 |
Target: 5'- gACGCUGUCAugGGCgaaaaa-CCCCCa -3' miRNA: 3'- -UGCGACAGUuuUUGauacuuaGGGGG- -5' |
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11736 | 3' | -46.3 | NC_003215.1 | + | 7085 | 1.15 | 0.000447 |
Target: 5'- gACGCUGUCAAAAACUAUGAAUCCCCCa -3' miRNA: 3'- -UGCGACAGUUUUUGAUACUUAGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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