Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11737 | 3' | -61.6 | NC_003215.1 | + | 10323 | 0.68 | 0.085977 |
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Target: 5'- gGGGGUacucaguucauauUCGGGCCGcUGuCAuCGGCCu -3' miRNA: 3'- gCCCCA-------------GGUCCGGCuACuGUcGCCGG- -5' |
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| 11737 | 3' | -61.6 | NC_003215.1 | + | 1865 | 0.68 | 0.076261 |
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Target: 5'- cCGGcGGUuaCCAGGUCGAcggauaUGACGGCagGGUCc -3' miRNA: 3'- -GCC-CCA--GGUCCGGCU------ACUGUCG--CCGG- -5' |
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| 11737 | 3' | -61.6 | NC_003215.1 | + | 7485 | 0.69 | 0.059523 |
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Target: 5'- aCGGGccgCCGGGCgCGGgcgccacCGGCGGCCg -3' miRNA: 3'- -GCCCca-GGUCCG-GCUacu----GUCGCCGG- -5' |
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| 11737 | 3' | -61.6 | NC_003215.1 | + | 4381 | 0.7 | 0.049362 |
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Target: 5'- uCGGcGG-CCAcuGcCCGGUaGACAGCGGCCa -3' miRNA: 3'- -GCC-CCaGGUc-C-GGCUA-CUGUCGCCGG- -5' |
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| 11737 | 3' | -61.6 | NC_003215.1 | + | 4326 | 0.71 | 0.046367 |
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Target: 5'- aGGGGaCCGcgaauuGGCCGcugucuaccgGGCAGUGGCCg -3' miRNA: 3'- gCCCCaGGU------CCGGCua--------CUGUCGCCGG- -5' |
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| 11737 | 3' | -61.6 | NC_003215.1 | + | 10382 | 1.1 | 0.000019 |
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Target: 5'- aCGGGGUCCAGGCCGAUGACAGCGGCCc -3' miRNA: 3'- -GCCCCAGGUCCGGCUACUGUCGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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