Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11738 | 3' | -50.9 | NC_003215.1 | + | 10957 | 0.66 | 0.487535 |
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Target: 5'- cGCUaugCAGUGCuAG-GGCCACuguUCAGCg -3' miRNA: 3'- -CGG---GUUACGuUCaCUGGUGuu-GGUCG- -5' |
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| 11738 | 3' | -50.9 | NC_003215.1 | + | 4774 | 0.66 | 0.487535 |
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Target: 5'- uGCCUccUGCAGucugGGCCACAACguCAGUa -3' miRNA: 3'- -CGGGuuACGUUca--CUGGUGUUG--GUCG- -5' |
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| 11738 | 3' | -50.9 | NC_003215.1 | + | 5349 | 0.66 | 0.440894 |
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Target: 5'- uCCCGcgcgGCgGAGcGACCGguGCCGGCg -3' miRNA: 3'- cGGGUua--CG-UUCaCUGGUguUGGUCG- -5' |
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| 11738 | 3' | -50.9 | NC_003215.1 | + | 5790 | 0.67 | 0.418541 |
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Target: 5'- cCCCGAUGUGAGccUGuCCACcACCguGGCu -3' miRNA: 3'- cGGGUUACGUUC--ACuGGUGuUGG--UCG- -5' |
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| 11738 | 3' | -50.9 | NC_003215.1 | + | 3598 | 0.69 | 0.327029 |
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Target: 5'- cGUCCugcugGUGAGUGAgUACAACCuGGCu -3' miRNA: 3'- -CGGGuua--CGUUCACUgGUGUUGG-UCG- -5' |
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| 11738 | 3' | -50.9 | NC_003215.1 | + | 9602 | 0.7 | 0.282967 |
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Target: 5'- aCUUAcUGCg---GGCCGCAACCAGCa -3' miRNA: 3'- cGGGUuACGuucaCUGGUGUUGGUCG- -5' |
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| 11738 | 3' | -50.9 | NC_003215.1 | + | 1283 | 0.71 | 0.236458 |
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Target: 5'- cGCCCAcuugcuaaAUGCGAc-GGCCACAAUCGGa -3' miRNA: 3'- -CGGGU--------UACGUUcaCUGGUGUUGGUCg -5' |
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| 11738 | 3' | -50.9 | NC_003215.1 | + | 11171 | 1.16 | 0.000091 |
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Target: 5'- aGCCCAAUGCAAGUGACCACAACCAGCa -3' miRNA: 3'- -CGGGUUACGUUCACUGGUGUUGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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