Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1176 | 5' | -50.6 | NC_001132.2 | + | 156727 | 1.04 | 0.018209 |
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Target: 5'- gAUACGGCGAACGAGACGAUCCGUUUUg -3' miRNA: 3'- -UAUGCCGCUUGCUCUGCUAGGCAAAA- -5' |
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| 1176 | 5' | -50.6 | NC_001132.2 | + | 48694 | 0.69 | 0.95431 |
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Target: 5'- -cGCGGUGAguuuauACGAGGCcuuGAUCUGUUUa -3' miRNA: 3'- uaUGCCGCU------UGCUCUG---CUAGGCAAAa -5' |
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| 1176 | 5' | -50.6 | NC_001132.2 | + | 9160 | 0.69 | 0.968718 |
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Target: 5'- cUACGaagauCGAACGAGGCGGUgCCGUg-- -3' miRNA: 3'- uAUGCc----GCUUGCUCUGCUA-GGCAaaa -5' |
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| 1176 | 5' | -50.6 | NC_001132.2 | + | 33427 | 0.68 | 0.977155 |
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Target: 5'- cGUACGGCaGAACGGGAC---CCGUg-- -3' miRNA: 3'- -UAUGCCG-CUUGCUCUGcuaGGCAaaa -5' |
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| 1176 | 5' | -50.6 | NC_001132.2 | + | 10647 | 0.68 | 0.977155 |
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Target: 5'- -cACGGCGAuCGuGGCGAacaaaCCGUUUa -3' miRNA: 3'- uaUGCCGCUuGCuCUGCUa----GGCAAAa -5' |
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| 1176 | 5' | -50.6 | NC_001132.2 | + | 34930 | 0.68 | 0.979555 |
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Target: 5'- uGUACGGCGAuagguCGgcAGAUGAUCCa---- -3' miRNA: 3'- -UAUGCCGCUu----GC--UCUGCUAGGcaaaa -5' |
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| 1176 | 5' | -50.6 | NC_001132.2 | + | 29663 | 0.66 | 0.992581 |
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Target: 5'- -aGCGGCGGACGAaccuccauUGGUCCGa--- -3' miRNA: 3'- uaUGCCGCUUGCUcu------GCUAGGCaaaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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