miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
118 3' -58 AC_000006.1 + 14833 0.66 0.431781
Target:  5'- gGAuGAGCUGGGAgUAGACgGCgaGGUc -3'
miRNA:   3'- -CUcCUCGACUCUgGUCUGgUGgaCCA- -5'
118 3' -58 AC_000006.1 + 10891 0.68 0.284903
Target:  5'- aGGGAGCUGcgcgaGGGCCuGGACCGCCa--- -3'
miRNA:   3'- cUCCUCGAC-----UCUGG-UCUGGUGGacca -5'
118 3' -58 AC_000006.1 + 14131 0.69 0.26348
Target:  5'- --uGGGCUGGGACCcuguGACCAagCUGGUg -3'
miRNA:   3'- cucCUCGACUCUGGu---CUGGUg-GACCA- -5'
118 3' -58 AC_000006.1 + 3278 1.08 0.000314
Target:  5'- cGAGGAGCUGAGACCAGACCACCUGGUg -3'
miRNA:   3'- -CUCCUCGACUCUGGUCUGGUGGACCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.