miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1180 3' -56.4 NC_001132.2 + 38719 0.66 0.929324
Target:  5'- aCGUGUCGGACUCGGuaaaGCAcacgGGCa -3'
miRNA:   3'- -GCACAGCCUGGGCCcaggCGUa---UUGg -5'
1180 3' -56.4 NC_001132.2 + 128297 0.66 0.924128
Target:  5'- uGUGUCGGAguccugUCCGGGacgucUCCuaAUGGCg -3'
miRNA:   3'- gCACAGCCU------GGGCCC-----AGGcgUAUUGg -5'
1180 3' -56.4 NC_001132.2 + 161221 0.7 0.743118
Target:  5'- aCGUcGUCGGAgaCCCGacgaacgaccauggaGGUCCGCGUAgaAUCg -3'
miRNA:   3'- -GCA-CAGCCU--GGGC---------------CCAGGCGUAU--UGG- -5'
1180 3' -56.4 NC_001132.2 + 2649 0.73 0.61124
Target:  5'- -aUG-CGGACCCGGGUCCGacacgGUAugUu -3'
miRNA:   3'- gcACaGCCUGGGCCCAGGCg----UAUugG- -5'
1180 3' -56.4 NC_001132.2 + 159308 1.12 0.002345
Target:  5'- cCGUGUCGGACCCGGGUCCGCAUAACCu -3'
miRNA:   3'- -GCACAGCCUGGGCCCAGGCGUAUUGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.