miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1181 3' -51.4 NC_001132.2 + 13804 0.66 0.993134
Target:  5'- --aCGGAAUACuaaaaugGUACCGAGGGa-- -3'
miRNA:   3'- gcaGCCUUAUGca-----CAUGGCUCCUgua -5'
1181 3' -51.4 NC_001132.2 + 150535 0.66 0.993134
Target:  5'- gCGUCGGAGUccuccacgACGgau-UCGGGGACGUc -3'
miRNA:   3'- -GCAGCCUUA--------UGCacauGGCUCCUGUA- -5'
1181 3' -51.4 NC_001132.2 + 8662 0.66 0.992105
Target:  5'- aGUCuGGAAccgACGUGUGCCGuGGcuCGg -3'
miRNA:   3'- gCAG-CCUUa--UGCACAUGGCuCCu-GUa -5'
1181 3' -51.4 NC_001132.2 + 9481 0.66 0.990957
Target:  5'- --cCGG-AUACGaccgaugGUACCGAGGACc- -3'
miRNA:   3'- gcaGCCuUAUGCa------CAUGGCUCCUGua -5'
1181 3' -51.4 NC_001132.2 + 2054 0.68 0.967986
Target:  5'- gCGUCGaGGgagacgccGUGCGUacuaUACCGAGGACGa -3'
miRNA:   3'- -GCAGC-CU--------UAUGCAc---AUGGCUCCUGUa -5'
1181 3' -51.4 NC_001132.2 + 161382 0.69 0.953648
Target:  5'- aCGUaGGAAUAgGUGUACCGucguacccucuGGACGa -3'
miRNA:   3'- -GCAgCCUUAUgCACAUGGCu----------CCUGUa -5'
1181 3' -51.4 NC_001132.2 + 159600 1.06 0.014523
Target:  5'- gCGUCGGAAUACGUGUACCGAGGACAUu -3'
miRNA:   3'- -GCAGCCUUAUGCACAUGGCUCCUGUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.