Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1182 | 5' | -55.1 | NC_001132.2 | + | 15775 | 0.66 | 0.958595 |
Target: 5'- cGUACGUCGCa---GCGCGUacucGUGcCCGa -3' miRNA: 3'- -CGUGCAGCGggcaUGCGCA----UACaGGC- -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 74172 | 0.66 | 0.958595 |
Target: 5'- cCACGUC-CUCGUACGCGUucgcgaacUCCa -3' miRNA: 3'- cGUGCAGcGGGCAUGCGCAuac-----AGGc -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 111003 | 0.66 | 0.958595 |
Target: 5'- uCAUGUgGUCCGcgACGUGUGUGauUCCu -3' miRNA: 3'- cGUGCAgCGGGCa-UGCGCAUAC--AGGc -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 133264 | 0.67 | 0.932899 |
Target: 5'- --cCGUCGCCCGUguuggaauaacACGUGUAcucguUGUCUu -3' miRNA: 3'- cguGCAGCGGGCA-----------UGCGCAU-----ACAGGc -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 1278 | 0.67 | 0.9225 |
Target: 5'- gGCGC-UCGCCCuacucgacACGUGUgaaGUGUCCGu -3' miRNA: 3'- -CGUGcAGCGGGca------UGCGCA---UACAGGC- -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 146332 | 0.68 | 0.905166 |
Target: 5'- aGgACG-CGUCCGUagcACGCGUAcagaUGUCUGu -3' miRNA: 3'- -CgUGCaGCGGGCA---UGCGCAU----ACAGGC- -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 157610 | 0.69 | 0.864459 |
Target: 5'- uCGCGaCGCCCGgACGUaGUuUGUCCGu -3' miRNA: 3'- cGUGCaGCGGGCaUGCG-CAuACAGGC- -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 127934 | 0.69 | 0.841302 |
Target: 5'- gGUAgGUCGCCCGgcaaGCGCGUAcagGUUa- -3' miRNA: 3'- -CGUgCAGCGGGCa---UGCGCAUa--CAGgc -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 103312 | 0.73 | 0.644883 |
Target: 5'- uGCACGUC-CCCccACGCGggAUGUCCc -3' miRNA: 3'- -CGUGCAGcGGGcaUGCGCa-UACAGGc -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 83474 | 0.74 | 0.594543 |
Target: 5'- uGCGCGUCGUCCGUccACGUGUAgGUUUu -3' miRNA: 3'- -CGUGCAGCGGGCA--UGCGCAUaCAGGc -5' |
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1182 | 5' | -55.1 | NC_001132.2 | + | 159564 | 1.12 | 0.003016 |
Target: 5'- aGCACGUCGCCCGUACGCGUAUGUCCGg -3' miRNA: 3'- -CGUGCAGCGGGCAUGCGCAUACAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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