Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11844 | 3' | -52.7 | NC_003266.2 | + | 11315 | 0.66 | 0.725349 |
Target: 5'- --cUGUGGGAccugcuGGAGGCCAuUGUGCa -3' miRNA: 3'- cgaACGCCCUuac---CCUUCGGU-GUACG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 5072 | 0.66 | 0.714094 |
Target: 5'- gGCgacUGCGGGAGUaGGgcGCCAgAcgauggGCg -3' miRNA: 3'- -CGa--ACGCCCUUAcCCuuCGGUgUa-----CG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 10839 | 0.66 | 0.712963 |
Target: 5'- uGCUguUGCGGuggaGggUGGGGGcgcaucuGCCGCAguacgggaUGCa -3' miRNA: 3'- -CGA--ACGCC----CuuACCCUU-------CGGUGU--------ACG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 27480 | 0.66 | 0.702748 |
Target: 5'- ----aCGGcGAuUGGGGAGCcCugGUGCa -3' miRNA: 3'- cgaacGCC-CUuACCCUUCG-GugUACG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 17153 | 0.68 | 0.621907 |
Target: 5'- gGCUgGCGGGAAgcGGGAugcgccGCCACccccaccgGCg -3' miRNA: 3'- -CGAaCGCCCUUa-CCCUu-----CGGUGua------CG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 20848 | 0.68 | 0.59872 |
Target: 5'- -gUUGCuGGAGaagGGGAugcGCCACAUGa -3' miRNA: 3'- cgAACGcCCUUa--CCCUu--CGGUGUACg -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 15513 | 0.68 | 0.574512 |
Target: 5'- cGCgugcGCGGGcAcuuccgcgcucccUGGGGcgcccucaagGGCCGCGUGCg -3' miRNA: 3'- -CGaa--CGCCCuU-------------ACCCU----------UCGGUGUACG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 23034 | 0.69 | 0.541481 |
Target: 5'- uUUUGCGGGGuuucuuuuUGGGcGGCUGC-UGCg -3' miRNA: 3'- cGAACGCCCUu-------ACCCuUCGGUGuACG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 22570 | 0.69 | 0.541481 |
Target: 5'- uCUUGUGGGcgaucUGGGAAugCGCAUGCa -3' miRNA: 3'- cGAACGCCCuu---ACCCUUcgGUGUACG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 12019 | 0.69 | 0.530233 |
Target: 5'- cGCaUUGCGGGAucaGGAGGUgGCuguUGCu -3' miRNA: 3'- -CG-AACGCCCUuacCCUUCGgUGu--ACG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 2936 | 0.69 | 0.519072 |
Target: 5'- uGCUgaccUGCGccGGUGGGAacAGUCAUAUGCu -3' miRNA: 3'- -CGA----ACGCccUUACCCU--UCGGUGUACG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 16851 | 0.71 | 0.403844 |
Target: 5'- aCUUGCGccGGggUuuucGGGgcGCCGCcgGCa -3' miRNA: 3'- cGAACGC--CCuuA----CCCuuCGGUGuaCG- -5' |
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11844 | 3' | -52.7 | NC_003266.2 | + | 3040 | 1.15 | 0.000375 |
Target: 5'- gGCUUGCGGGAAUGGGAAGCCACAUGCa -3' miRNA: 3'- -CGAACGCCCUUACCCUUCGGUGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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