Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11846 | 5' | -57.7 | NC_003266.2 | + | 13497 | 0.66 | 0.435917 |
Target: 5'- gGGGaaguucuUCUCccGCUcGGGCC-UCAACAGCg -3' miRNA: 3'- -UCC-------GGAGcuCGA-CCCGGuAGUUGUCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 21068 | 0.67 | 0.388989 |
Target: 5'- cGGGCUccggCGAGCaggagcucaGGGCCAUCAuCcGCg -3' miRNA: 3'- -UCCGGa---GCUCGa--------CCCGGUAGUuGuCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 23019 | 0.67 | 0.361941 |
Target: 5'- gAGGCCgaggagGAGCUGgcGGCCGUgGGCuucAGCu -3' miRNA: 3'- -UCCGGag----CUCGAC--CCGGUAgUUG---UCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 18068 | 0.67 | 0.361941 |
Target: 5'- cGGUCgcgcgCGGGCcacGGCCAcCGGCGGCa -3' miRNA: 3'- uCCGGa----GCUCGac-CCGGUaGUUGUCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 11725 | 0.68 | 0.336227 |
Target: 5'- cAGGCggcgCGAGCUGaGCgAUCAGgAGCu -3' miRNA: 3'- -UCCGga--GCUCGACcCGgUAGUUgUCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 24592 | 0.69 | 0.296394 |
Target: 5'- cGGGCCUCcccGCgcaGGGUgGUguGCAGCa -3' miRNA: 3'- -UCCGGAGcu-CGa--CCCGgUAguUGUCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 23823 | 0.69 | 0.288881 |
Target: 5'- gAGGCg-UGGGUUGGGCUcgCAGguGCc -3' miRNA: 3'- -UCCGgaGCUCGACCCGGuaGUUguCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 10876 | 0.69 | 0.281518 |
Target: 5'- cGGCCgccgUGAGCgGGGCCGggCAGaguCAGUa -3' miRNA: 3'- uCCGGa---GCUCGaCCCGGUa-GUU---GUCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 13360 | 0.71 | 0.221859 |
Target: 5'- cGGCCUCGGGCaccGCUG-CGGCAGCa -3' miRNA: 3'- uCCGGAGCUCGaccCGGUaGUUGUCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 12120 | 0.72 | 0.178197 |
Target: 5'- aAGGCUUCGGGgUugcGGGUCGUCAGC-GCc -3' miRNA: 3'- -UCCGGAGCUCgA---CCCGGUAGUUGuCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 11489 | 0.73 | 0.146429 |
Target: 5'- cGGcCCUCGGGCUcGGUgAuauUCAGCAGCg -3' miRNA: 3'- uCC-GGAGCUCGAcCCGgU---AGUUGUCG- -5' |
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11846 | 5' | -57.7 | NC_003266.2 | + | 3813 | 1.12 | 0.000169 |
Target: 5'- aAGGCCUCGAGCUGGGCCAUCAACAGCa -3' miRNA: 3'- -UCCGGAGCUCGACCCGGUAGUUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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