Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11847 | 3' | -50.9 | NC_003266.2 | + | 9227 | 0.66 | 0.796511 |
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Target: 5'- gGCGGGg----GAGGGGGcCUGCGUCg -3' miRNA: 3'- aCGUCUagacaCUCCCUUuGAUGCGG- -5' |
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| 11847 | 3' | -50.9 | NC_003266.2 | + | 4784 | 0.67 | 0.786073 |
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Target: 5'- cUGCAGGUgguaguUGAGGGAGACacaGCuGCCg -3' miRNA: 3'- -ACGUCUAgac---ACUCCCUUUGa--UG-CGG- -5' |
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| 11847 | 3' | -50.9 | NC_003266.2 | + | 717 | 0.67 | 0.775454 |
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Target: 5'- uUGUAuGAUCUG-GAGGuGGAugUGC-CCg -3' miRNA: 3'- -ACGU-CUAGACaCUCC-CUUugAUGcGG- -5' |
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| 11847 | 3' | -50.9 | NC_003266.2 | + | 18379 | 0.67 | 0.775454 |
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Target: 5'- aGUAGGugUCUGUGGcgcGGGcGAACUGCaCCa -3' miRNA: 3'- aCGUCU--AGACACU---CCC-UUUGAUGcGG- -5' |
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| 11847 | 3' | -50.9 | NC_003266.2 | + | 34822 | 0.68 | 0.731425 |
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Target: 5'- aGCAGaAUCUGUuggaauuauGAGGGuguGCUuaagaAUGCCg -3' miRNA: 3'- aCGUC-UAGACA---------CUCCCuu-UGA-----UGCGG- -5' |
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| 11847 | 3' | -50.9 | NC_003266.2 | + | 33643 | 0.68 | 0.708676 |
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Target: 5'- gGCAGuguguUCUG-GAGGGG----ACGCCa -3' miRNA: 3'- aCGUCu----AGACaCUCCCUuugaUGCGG- -5' |
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| 11847 | 3' | -50.9 | NC_003266.2 | + | 21308 | 0.68 | 0.697166 |
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Target: 5'- aGCAaAUCUaccaguucgaGUacGAGGGAcuGCUGCGCCg -3' miRNA: 3'- aCGUcUAGA----------CA--CUCCCUu-UGAUGCGG- -5' |
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| 11847 | 3' | -50.9 | NC_003266.2 | + | 17146 | 0.68 | 0.685585 |
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Target: 5'- cGUAGAaggCUG-GcGGGAAGCgggaUGCGCCg -3' miRNA: 3'- aCGUCUa--GACaCuCCCUUUG----AUGCGG- -5' |
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| 11847 | 3' | -50.9 | NC_003266.2 | + | 4659 | 1.12 | 0.000937 |
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Target: 5'- aUGCAGAUCUGUGAGGGAAACUACGCCc -3' miRNA: 3'- -ACGUCUAGACACUCCCUUUGAUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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