Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11849 | 5' | -58.5 | NC_003266.2 | + | 25337 | 0.66 | 0.448791 |
Target: 5'- aUUCUUgcUGAaaaAGGGCCGCGGgGUCu -3' miRNA: 3'- -AGGAGauGCUgc-UCCUGGCGCCgCAG- -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 25956 | 0.66 | 0.429363 |
Target: 5'- aUCCacagCggcgGCGGCggcagguggacuGAGGAUCGCGGCGa- -3' miRNA: 3'- -AGGa---Ga---UGCUG------------CUCCUGGCGCCGCag -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 22730 | 0.67 | 0.401172 |
Target: 5'- aCCUgUACaucaACGAGGAgcaCCGCGGCa-- -3' miRNA: 3'- aGGAgAUGc---UGCUCCU---GGCGCCGcag -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 23045 | 0.67 | 0.383045 |
Target: 5'- uUCUUUUugGGCGGcuGCUGCGGCGg- -3' miRNA: 3'- -AGGAGAugCUGCUccUGGCGCCGCag -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 17898 | 0.67 | 0.383045 |
Target: 5'- cUCCUCgUACGGgGGcGGuucguCCGUGuGCGUCa -3' miRNA: 3'- -AGGAG-AUGCUgCU-CCu----GGCGC-CGCAG- -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 11922 | 0.68 | 0.356895 |
Target: 5'- cUCUUCUACGuaaGGGACCGCcGCGg- -3' miRNA: 3'- -AGGAGAUGCugcUCCUGGCGcCGCag -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 15535 | 0.68 | 0.332024 |
Target: 5'- cUCC-CUGgGGCGcccucaAGGGCCGCGuGCGgUCg -3' miRNA: 3'- -AGGaGAUgCUGC------UCCUGGCGC-CGC-AG- -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 15396 | 0.68 | 0.324022 |
Target: 5'- gCCUUguaGACGuaGGGGCagguGCGGCGUCu -3' miRNA: 3'- aGGAGaugCUGC--UCCUGg---CGCCGCAG- -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 16999 | 0.69 | 0.293457 |
Target: 5'- -gCUgUGCGGCGAcGACgGCGGCGg- -3' miRNA: 3'- agGAgAUGCUGCUcCUGgCGCCGCag -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 14743 | 0.69 | 0.272043 |
Target: 5'- gCCUCUAC--CGAGGuCCGgGGCGa- -3' miRNA: 3'- aGGAGAUGcuGCUCCuGGCgCCGCag -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 23128 | 0.7 | 0.245467 |
Target: 5'- uUCUUCUugGucCGAGGcCaCGCGGCGg- -3' miRNA: 3'- -AGGAGAugCu-GCUCCuG-GCGCCGCag -5' |
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11849 | 5' | -58.5 | NC_003266.2 | + | 5689 | 1.09 | 0.000333 |
Target: 5'- uUCCUCUACGACGAGGACCGCGGCGUCc -3' miRNA: 3'- -AGGAGAUGCUGCUCCUGGCGCCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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