Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11850 | 5' | -57.9 | NC_003266.2 | + | 8991 | 0.66 | 0.444179 |
Target: 5'- aUUGCCAccacGCUcaacuaCCUCUaCCAGCGCCuCUg -3' miRNA: 3'- -AGCGGUu---CGA------GGAGA-GGUUGCGG-GAg -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 31167 | 0.67 | 0.368596 |
Target: 5'- aUCGCCucuccuacGAGCUCCUgCagCAGCGCCa-- -3' miRNA: 3'- -AGCGG--------UUCGAGGA-GagGUUGCGGgag -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 5457 | 0.68 | 0.342617 |
Target: 5'- aCGCCcccGAGUccgucuUCCUCgcccCCAagcucuACGCCCUCa -3' miRNA: 3'- aGCGG---UUCG------AGGAGa---GGU------UGCGGGAG- -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 15375 | 0.68 | 0.334256 |
Target: 5'- gCGCCGcGCgUCCUCUCgAGcCGCaCCUUc -3' miRNA: 3'- aGCGGUuCG-AGGAGAGgUU-GCG-GGAG- -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 20246 | 0.68 | 0.334256 |
Target: 5'- cUCGCUggGCUCCgggUUCGAC-CCCUa -3' miRNA: 3'- -AGCGGuuCGAGGag-AGGUUGcGGGAg -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 15631 | 0.68 | 0.326045 |
Target: 5'- cCGCCGcGC-CCgUCUCCAccguggACGCCgUCa -3' miRNA: 3'- aGCGGUuCGaGG-AGAGGU------UGCGGgAG- -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 9010 | 0.68 | 0.310075 |
Target: 5'- gUCGCCcaGGGCUUCcaagcgCUCCAugGCCUc- -3' miRNA: 3'- -AGCGG--UUCGAGGa-----GAGGUugCGGGag -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 8837 | 0.69 | 0.27994 |
Target: 5'- aCGCCuucucgcAGCUCaugggccgcaUCUCCAACGaCCUCg -3' miRNA: 3'- aGCGGu------UCGAGg---------AGAGGUUGCgGGAG- -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 25048 | 0.69 | 0.27278 |
Target: 5'- cUCGagCAGGC-CCUCgcugCUGACGUCCUCg -3' miRNA: 3'- -AGCg-GUUCGaGGAGa---GGUUGCGGGAG- -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 6721 | 0.69 | 0.265767 |
Target: 5'- -aGCCGGGCUCCUCgCC----CCCUCg -3' miRNA: 3'- agCGGUUCGAGGAGaGGuugcGGGAG- -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 4751 | 0.7 | 0.245605 |
Target: 5'- cCGCCcAGCUCaUCUCCccCGCCC-Cg -3' miRNA: 3'- aGCGGuUCGAGgAGAGGuuGCGGGaG- -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 15516 | 0.7 | 0.23288 |
Target: 5'- gUGCgCGGGCacuUCCgcgCUCCcuggGGCGCCCUCa -3' miRNA: 3'- aGCG-GUUCG---AGGa--GAGG----UUGCGGGAG- -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 22962 | 0.72 | 0.177362 |
Target: 5'- cCGCC-AGCUCCuccucggccUCUCUuuCGUCCUCg -3' miRNA: 3'- aGCGGuUCGAGG---------AGAGGuuGCGGGAG- -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 6885 | 0.75 | 0.103428 |
Target: 5'- gUCGCCAAGCUgCaagaCUCCuACGCCCa- -3' miRNA: 3'- -AGCGGUUCGAgGa---GAGGuUGCGGGag -5' |
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11850 | 5' | -57.9 | NC_003266.2 | + | 6156 | 1.1 | 0.000248 |
Target: 5'- aUCGCCAAGCUCCUCUCCAACGCCCUCu -3' miRNA: 3'- -AGCGGUUCGAGGAGAGGUUGCGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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