Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11851 | 5' | -55.7 | NC_003266.2 | + | 12439 | 0.66 | 0.530256 |
Target: 5'- aCUUCAUuagCGCCCUGCGgcuaauggUGACCGaGGuGCc -3' miRNA: 3'- -GGAGUA---GUGGGACGU--------AUUGGC-CC-CGa -5' |
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11851 | 5' | -55.7 | NC_003266.2 | + | 11752 | 0.68 | 0.4561 |
Target: 5'- gCUgAUgCACagCCUGCAgcgggcccUGACCGGGGCc -3' miRNA: 3'- gGAgUA-GUG--GGACGU--------AUUGGCCCCGa -5' |
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11851 | 5' | -55.7 | NC_003266.2 | + | 14554 | 0.68 | 0.444949 |
Target: 5'- gCCUCGUugguguaCACCCcggGCAUGACCagcucggucacGGGGUc -3' miRNA: 3'- -GGAGUA-------GUGGGa--CGUAUUGG-----------CCCCGa -5' |
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11851 | 5' | -55.7 | NC_003266.2 | + | 13448 | 0.68 | 0.426056 |
Target: 5'- uCCUCcUCGCCCaGCA-AGCgCGGGcGCg -3' miRNA: 3'- -GGAGuAGUGGGaCGUaUUG-GCCC-CGa -5' |
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11851 | 5' | -55.7 | NC_003266.2 | + | 12939 | 0.69 | 0.406693 |
Target: 5'- uCCUCggCGCUCaGCGcguACUGGGGCg -3' miRNA: 3'- -GGAGuaGUGGGaCGUau-UGGCCCCGa -5' |
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11851 | 5' | -55.7 | NC_003266.2 | + | 23492 | 0.69 | 0.397221 |
Target: 5'- uCCUcCAUC-UCUUGCAUGuCUGGGGCc -3' miRNA: 3'- -GGA-GUAGuGGGACGUAUuGGCCCCGa -5' |
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11851 | 5' | -55.7 | NC_003266.2 | + | 24683 | 0.7 | 0.360783 |
Target: 5'- aCCUCAagGCCCUGUG-GACCGGGu-- -3' miRNA: 3'- -GGAGUagUGGGACGUaUUGGCCCcga -5' |
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11851 | 5' | -55.7 | NC_003266.2 | + | 17245 | 0.7 | 0.343458 |
Target: 5'- cCCaUCAUCGCCgCgGCG--AUCGGGGCg -3' miRNA: 3'- -GG-AGUAGUGG-GaCGUauUGGCCCCGa -5' |
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11851 | 5' | -55.7 | NC_003266.2 | + | 25460 | 0.75 | 0.167344 |
Target: 5'- uCCUCGgggCAUCCUGgGggaAGCCGGGGUUa -3' miRNA: 3'- -GGAGUa--GUGGGACgUa--UUGGCCCCGA- -5' |
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11851 | 5' | -55.7 | NC_003266.2 | + | 6317 | 1.1 | 0.000398 |
Target: 5'- aCCUCAUCACCCUGCAUAACCGGGGCUg -3' miRNA: 3'- -GGAGUAGUGGGACGUAUUGGCCCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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