Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11852 | 3' | -53.5 | NC_003266.2 | + | 23678 | 0.69 | 0.52573 |
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Target: 5'- ---gUGCCccucaGCGUgGAGGAGCUCAGCCg -3' miRNA: 3'- gcagAUGG-----CGCGgCUUUUUGGGUCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 23800 | 0.69 | 0.547818 |
|
Target: 5'- gGUCUucGCgGUGCCc-GAGGCCCuGGCCa -3' miRNA: 3'- gCAGA--UGgCGCGGcuUUUUGGG-UCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 24040 | 0.66 | 0.683721 |
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Target: 5'- gCGUUUGCgGC-CCGAGucucgugcuGCCCAGaCCc -3' miRNA: 3'- -GCAGAUGgCGcGGCUUuu-------UGGGUC-GG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 24524 | 0.69 | 0.514811 |
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Target: 5'- aCGUgCUGCacaccacccUGCGCgGGGAGGCCC-GCCg -3' miRNA: 3'- -GCA-GAUG---------GCGCGgCUUUUUGGGuCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 26408 | 0.67 | 0.672415 |
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Target: 5'- aGUCcuggGCgGCGCCGGc-GGCCaGGCCc -3' miRNA: 3'- gCAGa---UGgCGCGGCUuuUUGGgUCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 27982 | 0.68 | 0.555619 |
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Target: 5'- cCGUCacuacaGCCGCGCUGcccAGACCCAaaaucgaaaguaccGCCa -3' miRNA: 3'- -GCAGa-----UGGCGCGGCuu-UUUGGGU--------------CGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 28215 | 0.66 | 0.706161 |
|
Target: 5'- uGUUUACCGUG-CGAagGAAGCCguCAGCa -3' miRNA: 3'- gCAGAUGGCGCgGCU--UUUUGG--GUCGg -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 35325 | 0.71 | 0.431317 |
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Target: 5'- -uUCUACaaugguCGCGCCG-GAGACCCuguAGCCu -3' miRNA: 3'- gcAGAUG------GCGCGGCuUUUUGGG---UCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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