Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11852 | 3' | -53.5 | NC_003266.2 | + | 5811 | 0.66 | 0.706162 |
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Target: 5'- aGUgUAggccaCGUGaCCGggGGuCCCGGCCg -3' miRNA: 3'- gCAgAUg----GCGC-GGCuuUUuGGGUCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 17138 | 0.66 | 0.683721 |
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Target: 5'- ----aACCGCGCCGuAGAAGgCUGGCg -3' miRNA: 3'- gcagaUGGCGCGGC-UUUUUgGGUCGg -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 6649 | 0.66 | 0.682593 |
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Target: 5'- aGUCaUACaacuCGUGCgagggggCGAGGAGCCCGGCUc -3' miRNA: 3'- gCAG-AUG----GCGCG-------GCUUUUUGGGUCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 13298 | 0.66 | 0.694974 |
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Target: 5'- gGUgCUGCCGCaGCgGu---GCCCgaGGCCg -3' miRNA: 3'- gCA-GAUGGCG-CGgCuuuuUGGG--UCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 9289 | 0.67 | 0.676943 |
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Target: 5'- gGUCUcGCCGCGCCGGcgucgcaugguCUCGGUg -3' miRNA: 3'- gCAGA-UGGCGCGGCUuuuu-------GGGUCGg -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 15450 | 0.67 | 0.669015 |
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Target: 5'- gGUCUGCgCGCGCCcagcaagauguacgGAGGcGCUC-GCCa -3' miRNA: 3'- gCAGAUG-GCGCGG--------------CUUUuUGGGuCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 3745 | 0.67 | 0.638301 |
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Target: 5'- aG-CUGCUGCuGuuGAu-GGCCCAGCUc -3' miRNA: 3'- gCaGAUGGCG-CggCUuuUUGGGUCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 21336 | 0.75 | 0.229047 |
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Target: 5'- --aCUGCUGCGCCGcAGcGCCCuGGCCa -3' miRNA: 3'- gcaGAUGGCGCGGCuUUuUGGG-UCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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