Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11853 | 3' | -51.9 | NC_003266.2 | + | 21773 | 0.66 | 0.793528 |
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Target: 5'- gCCgGGAGGUuucggCAUGGcccgcgggCAGGGACACg- -3' miRNA: 3'- -GGaUUUCCA-----GUACCa-------GUCCCUGUGga -5' |
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| 11853 | 3' | -51.9 | NC_003266.2 | + | 27396 | 0.67 | 0.751093 |
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Target: 5'- -gUGAAGGUCAgagacUGGUUgAGGGAUGCa- -3' miRNA: 3'- ggAUUUCCAGU-----ACCAG-UCCCUGUGga -5' |
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| 11853 | 3' | -51.9 | NC_003266.2 | + | 8444 | 0.67 | 0.740115 |
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Target: 5'- aCCcGGAGGcaGUGGUggCAGGGGCACg- -3' miRNA: 3'- -GGaUUUCCagUACCA--GUCCCUGUGga -5' |
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| 11853 | 3' | -51.9 | NC_003266.2 | + | 28983 | 0.67 | 0.71781 |
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Target: 5'- aCUGGGGGUgGUaGG-CAGaGGACugCUg -3' miRNA: 3'- gGAUUUCCAgUA-CCaGUC-CCUGugGA- -5' |
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| 11853 | 3' | -51.9 | NC_003266.2 | + | 33345 | 0.73 | 0.360462 |
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Target: 5'- aCUcaAGGGGUCcgGGUcCAGGGACucCCUg -3' miRNA: 3'- gGA--UUUCCAGuaCCA-GUCCCUGu-GGA- -5' |
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| 11853 | 3' | -51.9 | NC_003266.2 | + | 7186 | 1.09 | 0.001269 |
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Target: 5'- uCCUAAAGGUCAUGGUCAGGGACACCUu -3' miRNA: 3'- -GGAUUUCCAGUACCAGUCCCUGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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