Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11855 | 5' | -59.9 | NC_003266.2 | + | 6584 | 0.66 | 0.381881 |
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Target: 5'- cCGCGCUCacccacCCCaUGCCC--UGGGGcacGCCc -3' miRNA: 3'- -GCGUGAGa-----GGG-ACGGGuuGCCCC---UGG- -5' |
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| 11855 | 5' | -59.9 | NC_003266.2 | + | 3553 | 0.66 | 0.373103 |
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Target: 5'- aCGCAC--UCCC-GCCCAGgaGGGGAg- -3' miRNA: 3'- -GCGUGagAGGGaCGGGUUg-CCCCUgg -5' |
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| 11855 | 5' | -59.9 | NC_003266.2 | + | 14220 | 0.66 | 0.355961 |
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Target: 5'- gGCGCgggucauggUCUCCCgcaagaccCCCAACGGGGugaCa -3' miRNA: 3'- gCGUG---------AGAGGGac------GGGUUGCCCCug-G- -5' |
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| 11855 | 5' | -59.9 | NC_003266.2 | + | 6370 | 0.67 | 0.310969 |
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Target: 5'- cCGC-CUCUgcuggaccaacgagcCCCUGCgCGGCGaGGuGGCCa -3' miRNA: 3'- -GCGuGAGA---------------GGGACGgGUUGC-CC-CUGG- -5' |
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| 11855 | 5' | -59.9 | NC_003266.2 | + | 5617 | 0.68 | 0.278747 |
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Target: 5'- aGC-CUCUUUgUcaCCCAGCGGGGACa -3' miRNA: 3'- gCGuGAGAGGgAc-GGGUUGCCCCUGg -5' |
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| 11855 | 5' | -59.9 | NC_003266.2 | + | 17181 | 0.68 | 0.258339 |
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Target: 5'- gGCGCgguuuUCUUCCUcgguaGCCCGuaAUGGGGACg -3' miRNA: 3'- gCGUG-----AGAGGGA-----CGGGU--UGCCCCUGg -5' |
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| 11855 | 5' | -59.9 | NC_003266.2 | + | 9365 | 0.7 | 0.209872 |
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Target: 5'- cCGCGCaUCUCCaggugGCCCGG-GGGGuCCc -3' miRNA: 3'- -GCGUG-AGAGGga---CGGGUUgCCCCuGG- -5' |
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| 11855 | 5' | -59.9 | NC_003266.2 | + | 21851 | 0.7 | 0.193818 |
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Target: 5'- cCGCAacgUgUCCCUGCCCG-CGGGccauGCCg -3' miRNA: 3'- -GCGUg--AgAGGGACGGGUuGCCCc---UGG- -5' |
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| 11855 | 5' | -59.9 | NC_003266.2 | + | 23190 | 0.77 | 0.059883 |
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Target: 5'- aCGgGCUCUCgCgGCCCGGCGGGuGGCUg -3' miRNA: 3'- -GCgUGAGAGgGaCGGGUUGCCC-CUGG- -5' |
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| 11855 | 5' | -59.9 | NC_003266.2 | + | 9451 | 1.1 | 0.000176 |
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Target: 5'- gCGCACUCUCCCUGCCCAACGGGGACCc -3' miRNA: 3'- -GCGUGAGAGGGACGGGUUGCCCCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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