Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11856 | 3' | -62.7 | NC_003266.2 | + | 3778 | 0.66 | 0.271454 |
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Target: 5'- uGACCcaGCGCCU-GGGCGa-GCUGACCc -3' miRNA: 3'- -CUGG--UGCGGAcCCCGUacCGGCUGGc -5' |
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| 11856 | 3' | -62.7 | NC_003266.2 | + | 6301 | 0.66 | 0.239167 |
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Target: 5'- cGCCGCGCa-GGGGCucguUGGUccagcagaggCGGCCGc -3' miRNA: 3'- cUGGUGCGgaCCCCGu---ACCG----------GCUGGC- -5' |
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| 11856 | 3' | -62.7 | NC_003266.2 | + | 10935 | 0.68 | 0.184223 |
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Target: 5'- gGGCUggcGCGCCUgGGGGCGUcGUCG-CCGg -3' miRNA: 3'- -CUGG---UGCGGA-CCCCGUAcCGGCuGGC- -5' |
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| 11856 | 3' | -62.7 | NC_003266.2 | + | 13551 | 0.68 | 0.184223 |
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Target: 5'- gGGCCACcaGCC-GGGGCAguGCCGcCCGu -3' miRNA: 3'- -CUGGUG--CGGaCCCCGUacCGGCuGGC- -5' |
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| 11856 | 3' | -62.7 | NC_003266.2 | + | 12462 | 0.7 | 0.136942 |
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Target: 5'- -uCCugGCCccGGGGCAcguUGGCgGGCUGc -3' miRNA: 3'- cuGGugCGGa-CCCCGU---ACCGgCUGGC- -5' |
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| 11856 | 3' | -62.7 | NC_003266.2 | + | 6786 | 0.73 | 0.080718 |
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Target: 5'- ---uGgGCgUGGGGCA-GGCCGACCGa -3' miRNA: 3'- cuggUgCGgACCCCGUaCCGGCUGGC- -5' |
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| 11856 | 3' | -62.7 | NC_003266.2 | + | 21204 | 0.73 | 0.080718 |
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Target: 5'- cGGCCGCGagaCCgGGGGCGagcauUGGCUGGCCu -3' miRNA: 3'- -CUGGUGC---GGaCCCCGU-----ACCGGCUGGc -5' |
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| 11856 | 3' | -62.7 | NC_003266.2 | + | 9741 | 1.07 | 0.00015 |
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Target: 5'- gGACCACGCCUGGGGCAUGGCCGACCGu -3' miRNA: 3'- -CUGGUGCGGACCCCGUACCGGCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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