Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11857 | 3' | -56.1 | NC_003266.2 | + | 13913 | 0.66 | 0.567745 |
Target: 5'- gCCGCCGcCGCCAUugccugcugcaucacGCUCucguacgagggagGAGGACCc-- -3' miRNA: 3'- -GGUGGC-GUGGUA---------------CGAG-------------CUCCUGGauc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 10869 | 0.66 | 0.564424 |
Target: 5'- aCCGCCGCggccGCCGUGagCG-GGGCCg-- -3' miRNA: 3'- -GGUGGCG----UGGUACgaGCuCCUGGauc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 27030 | 0.67 | 0.487797 |
Target: 5'- gCCGCCGCuuucgcuGCUucGCUCG-GGACCUc- -3' miRNA: 3'- -GGUGGCG-------UGGuaCGAGCuCCUGGAuc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 16144 | 0.67 | 0.478418 |
Target: 5'- gCCGCCGCgggGCCGUagaucucagGCgCGAuGACCUGGa -3' miRNA: 3'- -GGUGGCG---UGGUA---------CGaGCUcCUGGAUC- -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 25183 | 0.67 | 0.468103 |
Target: 5'- aCUugCGCAaguUCGUGCcCGAGGACUa-- -3' miRNA: 3'- -GGugGCGU---GGUACGaGCUCCUGGauc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 17894 | 0.67 | 0.468103 |
Target: 5'- cCCAUUGCGCCucuaGCUacCGGGGugCUGa -3' miRNA: 3'- -GGUGGCGUGGua--CGA--GCUCCugGAUc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 4379 | 0.68 | 0.457904 |
Target: 5'- gCACCGgauaCACCGUGCUgGuGGuCCUGc -3' miRNA: 3'- gGUGGC----GUGGUACGAgCuCCuGGAUc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 19811 | 0.68 | 0.428042 |
Target: 5'- gCUACCGCuCCAUGCUCcuGGGCaacGGg -3' miRNA: 3'- -GGUGGCGuGGUACGAGcuCCUGga-UC- -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 20053 | 0.68 | 0.418346 |
Target: 5'- aCACCGCcuCCAcGCUUGAGG-CCa-- -3' miRNA: 3'- gGUGGCGu-GGUaCGAGCUCCuGGauc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 24347 | 0.68 | 0.418346 |
Target: 5'- gCGCCGCucuuCCAaGCUCuGGGGACUa-- -3' miRNA: 3'- gGUGGCGu---GGUaCGAG-CUCCUGGauc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 3642 | 0.68 | 0.408786 |
Target: 5'- gCCGCCaGCGCCGUGCgCGGaauGGCCaUGGg -3' miRNA: 3'- -GGUGG-CGUGGUACGaGCUc--CUGG-AUC- -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 5674 | 0.68 | 0.408786 |
Target: 5'- -gACCGCGgCGUcccaCUCGAGGACCg-- -3' miRNA: 3'- ggUGGCGUgGUAc---GAGCUCCUGGauc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 7462 | 0.69 | 0.380938 |
Target: 5'- gUCGCUGaGCCcgugcUGCUCGAGGGCCcAGu -3' miRNA: 3'- -GGUGGCgUGGu----ACGAGCUCCUGGaUC- -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 33723 | 0.69 | 0.371941 |
Target: 5'- cCCGCC-CGCCAUGCagCGcaGGGACCc-- -3' miRNA: 3'- -GGUGGcGUGGUACGa-GC--UCCUGGauc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 18422 | 0.69 | 0.36309 |
Target: 5'- aCGCUGCGCUuuGUGCcCGuGGACCgggAGg -3' miRNA: 3'- gGUGGCGUGG--UACGaGCuCCUGGa--UC- -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 18534 | 0.7 | 0.345831 |
Target: 5'- aCAuCCGCGgCGUGCuggaUCGGGGcCCUAGc -3' miRNA: 3'- gGU-GGCGUgGUACG----AGCUCCuGGAUC- -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 33456 | 0.75 | 0.156103 |
Target: 5'- cCCGCCGUACCAggcgacugaUGCUC-AGGGCCa-- -3' miRNA: 3'- -GGUGGCGUGGU---------ACGAGcUCCUGGauc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 24306 | 0.77 | 0.124469 |
Target: 5'- gCCACCGCGCCAgcUGCUCGucgcuGACCa-- -3' miRNA: 3'- -GGUGGCGUGGU--ACGAGCuc---CUGGauc -5' |
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11857 | 3' | -56.1 | NC_003266.2 | + | 10194 | 1.1 | 0.000426 |
Target: 5'- aCCACCGCACCAUGCUCGAGGACCUAGc -3' miRNA: 3'- -GGUGGCGUGGUACGAGCUCCUGGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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