Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11859 | 3' | -63.4 | NC_003266.2 | + | 7413 | 0.66 | 0.250829 |
Target: 5'- cCuuGACGUGGGGcaGUuucuugagcuccucGUAGGUGAGCu- -3' miRNA: 3'- -GggCUGCGCCCC--CG--------------CGUCCACUCGcu -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 23403 | 0.66 | 0.240371 |
Target: 5'- gUCGGCgGUGGGGGCagaugGCgAGGUuGGCGAc -3' miRNA: 3'- gGGCUG-CGCCCCCG-----CG-UCCAcUCGCU- -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 5305 | 0.68 | 0.190944 |
Target: 5'- gCCGACGCacaGGGCGCcgaucGG-GAGCGGu -3' miRNA: 3'- gGGCUGCGcc-CCCGCGu----CCaCUCGCU- -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 9553 | 0.68 | 0.18604 |
Target: 5'- uUCUGGCgGCGguGGGGUGUGGGcggGGGCGAu -3' miRNA: 3'- -GGGCUG-CGC--CCCCGCGUCCa--CUCGCU- -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 15602 | 0.68 | 0.180774 |
Target: 5'- aCCGcACGCGGcccuugaGGGCGCcccAGG-GAGCGc -3' miRNA: 3'- gGGC-UGCGCC-------CCCGCG---UCCaCUCGCu -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 18026 | 0.69 | 0.163154 |
Target: 5'- aCUGugGa-GGGGCGgGGaGUGGGCGGa -3' miRNA: 3'- gGGCugCgcCCCCGCgUC-CACUCGCU- -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 30512 | 0.69 | 0.146702 |
Target: 5'- aCUgGGgGUGGuGGCGCAGGUaGGCGAu -3' miRNA: 3'- -GGgCUgCGCCcCCGCGUCCAcUCGCU- -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 5407 | 0.7 | 0.130715 |
Target: 5'- aCgGACuCGGGGGCGUAGGcguccgcgccgcagUGGGCGc -3' miRNA: 3'- gGgCUGcGCCCCCGCGUCC--------------ACUCGCu -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 22431 | 0.71 | 0.111999 |
Target: 5'- aCCCaGAacCGCuGGGGGCGCAGcGUGGuGCa- -3' miRNA: 3'- -GGG-CU--GCG-CCCCCGCGUC-CACU-CGcu -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 5834 | 0.71 | 0.108986 |
Target: 5'- uCCCGGC-CGGGGGgGUauaaaAGG-GGGCGGg -3' miRNA: 3'- -GGGCUGcGCCCCCgCG-----UCCaCUCGCU- -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 10110 | 0.71 | 0.103185 |
Target: 5'- cUCgGugGCGGGGGCGCcgggcgcuAGGUccucGAGCa- -3' miRNA: 3'- -GGgCugCGCCCCCGCG--------UCCA----CUCGcu -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 15678 | 0.71 | 0.103185 |
Target: 5'- aCgGGCGCGGcGGCGgGGGUGuaguuGCGAg -3' miRNA: 3'- gGgCUGCGCCcCCGCgUCCACu----CGCU- -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 15277 | 0.72 | 0.097408 |
Target: 5'- aCUGACGgugguaaUGGuGGGCGCGGGcGGGCGGa -3' miRNA: 3'- gGGCUGC-------GCC-CCCGCGUCCaCUCGCU- -5' |
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11859 | 3' | -63.4 | NC_003266.2 | + | 13747 | 1.09 | 0.000102 |
Target: 5'- gCCCGACGCGGGGGCGCAGGUGAGCGAa -3' miRNA: 3'- -GGGCUGCGCCCCCGCGUCCACUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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