Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11859 | 5' | -57.4 | NC_003266.2 | + | 14736 | 0.66 | 0.472823 |
Target: 5'- aGCCACCGC-CUCuaCCGAgGUCCGGg -3' miRNA: 3'- gUGGUGGUGaGGGcgGGUU-CAGGUUg -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 4872 | 0.66 | 0.462548 |
Target: 5'- uCGCgCACCAgUUCCGCCagGAGUcgcucuccgcCCAGCg -3' miRNA: 3'- -GUG-GUGGUgAGGGCGGg-UUCA----------GGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 10822 | 0.66 | 0.452392 |
Target: 5'- aGCCGgCGCUUCUGCCUccGccCCAGCa -3' miRNA: 3'- gUGGUgGUGAGGGCGGGuuCa-GGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 6293 | 0.66 | 0.452392 |
Target: 5'- gGCCACC--UCgCCGCgCAggggcucguuGGUCCAGCa -3' miRNA: 3'- gUGGUGGugAG-GGCGgGU----------UCAGGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 27150 | 0.66 | 0.449369 |
Target: 5'- -cCCACCuGCUUCguaucuucagccagCGCCCGauccuGGUCCAGCg -3' miRNA: 3'- guGGUGG-UGAGG--------------GCGGGU-----UCAGGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 10196 | 0.67 | 0.422677 |
Target: 5'- cUACCACCGCaCCaUGCUCGAGgacCUAGCg -3' miRNA: 3'- -GUGGUGGUGaGG-GCGGGUUCa--GGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 33338 | 0.67 | 0.422677 |
Target: 5'- aGCUGCUACUCaagggGUCCGGGUCCAGg -3' miRNA: 3'- gUGGUGGUGAGgg---CGGGUUCAGGUUg -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 30848 | 0.67 | 0.422677 |
Target: 5'- gCACCACCAC-CacaGCCUAGGccCCAAUa -3' miRNA: 3'- -GUGGUGGUGaGgg-CGGGUUCa-GGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 4281 | 0.67 | 0.413035 |
Target: 5'- uUACCGCCcagaUCCCGCCUggGaUUCAu- -3' miRNA: 3'- -GUGGUGGug--AGGGCGGGuuC-AGGUug -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 26351 | 0.67 | 0.40353 |
Target: 5'- gGCCGCCgGCg-CCGCCCAGGacuacUCCAcccGCa -3' miRNA: 3'- gUGGUGG-UGagGGCGGGUUC-----AGGU---UG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 11139 | 0.67 | 0.40353 |
Target: 5'- gCGCCGCaGCUCCCGCCCcgcGUggaAACg -3' miRNA: 3'- -GUGGUGgUGAGGGCGGGuu-CAgg-UUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 8153 | 0.67 | 0.40353 |
Target: 5'- gACCugC-CUCCCGCgCGGGcucgUCAGCa -3' miRNA: 3'- gUGGugGuGAGGGCGgGUUCa---GGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 23728 | 0.67 | 0.394164 |
Target: 5'- -cUCGCCGCgcgugCCC-CCCAAGcgCCAGCc -3' miRNA: 3'- guGGUGGUGa----GGGcGGGUUCa-GGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 15037 | 0.67 | 0.394164 |
Target: 5'- uCACCuuCCGCUCCaCGCgUCAAGUUagCAACu -3' miRNA: 3'- -GUGGu-GGUGAGG-GCG-GGUUCAG--GUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 30776 | 0.67 | 0.384941 |
Target: 5'- aACgCACCcgaggcgcucugGCUCCCGCCUGAucCCGACa -3' miRNA: 3'- gUG-GUGG------------UGAGGGCGGGUUcaGGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 15919 | 0.67 | 0.384941 |
Target: 5'- uGCCGCCGCcguggCCgCGCgucuCCGAGUCCuGCc -3' miRNA: 3'- gUGGUGGUGa----GG-GCG----GGUUCAGGuUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 26555 | 0.68 | 0.348648 |
Target: 5'- gACCguACUACUUCCgcgagacgcccagGCCgAAGUCCAGCu -3' miRNA: 3'- gUGG--UGGUGAGGG-------------CGGgUUCAGGUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 7403 | 0.68 | 0.348648 |
Target: 5'- aACgGCUucgacgagaucguGCUCgCCGCCCAGGUCauCAACa -3' miRNA: 3'- gUGgUGG-------------UGAG-GGCGGGUUCAG--GUUG- -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 8423 | 0.69 | 0.316451 |
Target: 5'- aGCCaaACCGCUUCCGCCCg---CCAAg -3' miRNA: 3'- gUGG--UGGUGAGGGCGGGuucaGGUUg -5' |
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11859 | 5' | -57.4 | NC_003266.2 | + | 3566 | 0.69 | 0.316451 |
Target: 5'- cCAUCACauuuugacgCACUCCCGCCCAGGaggggaGACg -3' miRNA: 3'- -GUGGUG---------GUGAGGGCGGGUUCagg---UUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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