miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1186 3' -51.2 NC_001132.2 + 33207 0.67 0.98533
Target:  5'- aCGACGuuGaGAUucuucguauauaucgAGGGGCGCGGGAGGa -3'
miRNA:   3'- -GUUGCucC-CUA---------------UCCUUGCGCUCUUCa -5'
1186 3' -51.2 NC_001132.2 + 10838 0.68 0.975048
Target:  5'- --cUGAGGGAcgcccuccagagacUGGGggUGCGAGAc-- -3'
miRNA:   3'- guuGCUCCCU--------------AUCCuuGCGCUCUuca -5'
1186 3' -51.2 NC_001132.2 + 44527 0.68 0.973153
Target:  5'- cCGACGAGGGGaaccaacgUAGGAuACGCGAc---- -3'
miRNA:   3'- -GUUGCUCCCU--------AUCCU-UGCGCUcuuca -5'
1186 3' -51.2 NC_001132.2 + 2149 0.68 0.967171
Target:  5'- gAACGAGcGGcagcgAGG-GCGCGGGAGGa -3'
miRNA:   3'- gUUGCUC-CCua---UCCuUGCGCUCUUCa -5'
1186 3' -51.2 NC_001132.2 + 20640 0.77 0.631561
Target:  5'- ---aGAGGGAcaaUGGGAACGCGGGGAa- -3'
miRNA:   3'- guugCUCCCU---AUCCUUGCGCUCUUca -5'
1186 3' -51.2 NC_001132.2 + 157221 1.08 0.009929
Target:  5'- cCAACGAGGGAUAGGAACGCGAGAAGUa -3'
miRNA:   3'- -GUUGCUCCCUAUCCUUGCGCUCUUCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.