Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11860 | 3' | -57.1 | NC_003266.2 | + | 16316 | 0.66 | 0.521404 |
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Target: 5'- aGCGCUCcuacgaugaggUGUACG-GGGAcGAGGAUa -3' miRNA: 3'- gUGCGAGa----------GCAUGCuCCCUcCUCCUG- -5' |
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| 11860 | 3' | -57.1 | NC_003266.2 | + | 14666 | 0.66 | 0.480277 |
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Target: 5'- cCGCGCUCUUGgauguCGAGGccuaugagaaaagcaAGGAGGAg -3' miRNA: 3'- -GUGCGAGAGCau---GCUCCc--------------UCCUCCUg -5' |
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| 11860 | 3' | -57.1 | NC_003266.2 | + | 12206 | 0.67 | 0.428051 |
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Target: 5'- gCACcUUCUCGUGCGuGGGGuuggagcGcGAGGGCa -3' miRNA: 3'- -GUGcGAGAGCAUGCuCCCU-------C-CUCCUG- -5' |
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| 11860 | 3' | -57.1 | NC_003266.2 | + | 35607 | 0.69 | 0.338614 |
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Target: 5'- gACGCUCagguuUUGUAUguguuucugGAGGGAGcGGGGGCu -3' miRNA: 3'- gUGCGAG-----AGCAUG---------CUCCCUC-CUCCUG- -5' |
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| 11860 | 3' | -57.1 | NC_003266.2 | + | 6639 | 0.77 | 0.092596 |
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Target: 5'- gCGCGCacguagucauacaaCUCGUGCGAGGGGGcGAGGAg -3' miRNA: 3'- -GUGCGa-------------GAGCAUGCUCCCUC-CUCCUg -5' |
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| 11860 | 3' | -57.1 | NC_003266.2 | + | 13888 | 1.08 | 0.000482 |
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Target: 5'- uCACGCUCUCGUACGAGGGAGGAGGACc -3' miRNA: 3'- -GUGCGAGAGCAUGCUCCCUCCUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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