Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11861 | 5' | -55.9 | NC_003266.2 | + | 21992 | 0.66 | 0.558101 |
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Target: 5'- aGGGUUgCAGCACUGGAa-CACcaUCa -3' miRNA: 3'- gCCCAGaGUCGUGGCCUgcGUGaaAG- -5' |
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| 11861 | 5' | -55.9 | NC_003266.2 | + | 8727 | 0.66 | 0.547038 |
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Target: 5'- -aGGUCUCcGCgGCCGG-CGCGCUc-- -3' miRNA: 3'- gcCCAGAGuCG-UGGCCuGCGUGAaag -5' |
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| 11861 | 5' | -55.9 | NC_003266.2 | + | 12106 | 0.67 | 0.503567 |
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Target: 5'- gCGGGUCgUCAGCGCCauGAUGCGu---- -3' miRNA: 3'- -GCCCAG-AGUCGUGGc-CUGCGUgaaag -5' |
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| 11861 | 5' | -55.9 | NC_003266.2 | + | 17966 | 0.68 | 0.431474 |
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Target: 5'- uCGGGUUUCAGCACCccgguagcuaGaGGCGCAa---- -3' miRNA: 3'- -GCCCAGAGUCGUGG----------C-CUGCGUgaaag -5' |
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| 11861 | 5' | -55.9 | NC_003266.2 | + | 9288 | 0.69 | 0.393082 |
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Target: 5'- -uGGUCUCGccGCGCCGGcguCGCAUggUCu -3' miRNA: 3'- gcCCAGAGU--CGUGGCCu--GCGUGaaAG- -5' |
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| 11861 | 5' | -55.9 | NC_003266.2 | + | 16303 | 1.09 | 0.00051 |
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Target: 5'- cCGGGUCUCAGCACCGGACGCACUUUCc -3' miRNA: 3'- -GCCCAGAGUCGUGGCCUGCGUGAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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