Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11862 | 5' | -59 | NC_003266.2 | + | 10184 | 0.66 | 0.408396 |
Target: 5'- aGGugauGCCgGCGGCgGUGGUggag-GCg -3' miRNA: 3'- gCCu---CGGgCGCCGgUACCGaagaaCG- -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 17777 | 0.66 | 0.399083 |
Target: 5'- aCGGAGCCgGCGGggggaaccggguCCAggcGGCUguugaUCUgccgcUGCa -3' miRNA: 3'- -GCCUCGGgCGCC------------GGUa--CCGA-----AGA-----ACG- -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 23181 | 0.66 | 0.399082 |
Target: 5'- gCGGAGgcgacgggcuCUCGCGGCCcggcggGUGGCUg---GCa -3' miRNA: 3'- -GCCUC----------GGGCGCCGG------UACCGAagaaCG- -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 6675 | 0.67 | 0.332904 |
Target: 5'- aGGAGCCCggcuccgagauuggcGCGGCUG-GGUUUUUcgGCg -3' miRNA: 3'- gCCUCGGG---------------CGCCGGUaCCGAAGAa-CG- -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 10920 | 0.67 | 0.329653 |
Target: 5'- aCGGcGgCCGCGGCgguaGUGGCUggaagUUGCu -3' miRNA: 3'- -GCCuCgGGCGCCGg---UACCGAag---AACG- -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 5177 | 0.68 | 0.290993 |
Target: 5'- aGGGGUgCGC-GCCG-GGCUgggcgCUUGCg -3' miRNA: 3'- gCCUCGgGCGcCGGUaCCGAa----GAACG- -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 15857 | 0.7 | 0.201718 |
Target: 5'- uCGGAGaCgCGCGGCCAcGGCggcg-GCa -3' miRNA: 3'- -GCCUC-GgGCGCCGGUaCCGaagaaCG- -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 26400 | 0.71 | 0.185983 |
Target: 5'- gCGGcGCCgGCGGCCA-GGCccaUCUgggGCu -3' miRNA: 3'- -GCCuCGGgCGCCGGUaCCGa--AGAa--CG- -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 4951 | 0.73 | 0.137221 |
Target: 5'- -uGAGCCCGuCGGCCAUgGGCaUUUUGg -3' miRNA: 3'- gcCUCGGGC-GCCGGUA-CCGaAGAACg -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 11440 | 0.73 | 0.126117 |
Target: 5'- -cGAGCCCGagGGCCGcUGGCUUCUg-- -3' miRNA: 3'- gcCUCGGGCg-CCGGU-ACCGAAGAacg -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 23009 | 0.8 | 0.039466 |
Target: 5'- aGGAGCUgGCGGCCGUgGGCUUCa-GCu -3' miRNA: 3'- gCCUCGGgCGCCGGUA-CCGAAGaaCG- -5' |
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11862 | 5' | -59 | NC_003266.2 | + | 21112 | 1.12 | 0.000138 |
Target: 5'- cCGGAGCCCGCGGCCAUGGCUUCUUGCa -3' miRNA: 3'- -GCCUCGGGCGCCGGUACCGAAGAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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