Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11864 | 5' | -60.7 | NC_003266.2 | + | 17000 | 0.66 | 0.346029 |
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Target: 5'- -aGCUGUGcgGCgacgACGGCGgCGGGUGg- -3' miRNA: 3'- gaCGACGCa-CG----UGCUGCgGCCCACgc -5' |
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| 11864 | 5' | -60.7 | NC_003266.2 | + | 11171 | 0.66 | 0.341093 |
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Target: 5'- cCUGCgcGCGcGCACGugGCCGcGGccaaccuggucacgGCGu -3' miRNA: 3'- -GACGa-CGCaCGUGCugCGGC-CCa-------------CGC- -5' |
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| 11864 | 5' | -60.7 | NC_003266.2 | + | 17245 | 0.66 | 0.329775 |
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Target: 5'- uUGCUGaugGCGCGcCGCCGccGGUGgGg -3' miRNA: 3'- gACGACgcaCGUGCuGCGGC--CCACgC- -5' |
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| 11864 | 5' | -60.7 | NC_003266.2 | + | 9806 | 0.67 | 0.298979 |
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Target: 5'- -cGCUGCG-GCugGacgaGCGCCaGGUcaGCGa -3' miRNA: 3'- gaCGACGCaCGugC----UGCGGcCCA--CGC- -5' |
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| 11864 | 5' | -60.7 | NC_003266.2 | + | 11094 | 0.67 | 0.284435 |
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Target: 5'- -aGCUGCG-GCGCGGC-CUggacagaaagaGGGUGCu -3' miRNA: 3'- gaCGACGCaCGUGCUGcGG-----------CCCACGc -5' |
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| 11864 | 5' | -60.7 | NC_003266.2 | + | 16495 | 0.68 | 0.225868 |
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Target: 5'- gUGCUGCcgaGCGCGGCGCCgcgucGGG-GCu -3' miRNA: 3'- gACGACGca-CGUGCUGCGG-----CCCaCGc -5' |
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| 11864 | 5' | -60.7 | NC_003266.2 | + | 5181 | 0.69 | 0.208747 |
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Target: 5'- gUGC-GCGccggGCugGGCGCUugcgaGGGUGCGc -3' miRNA: 3'- gACGaCGCa---CGugCUGCGG-----CCCACGC- -5' |
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| 11864 | 5' | -60.7 | NC_003266.2 | + | 13038 | 0.7 | 0.179779 |
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Target: 5'- aUGUUGCGcgcggucaugucgaGCGCGGCGCUGGGgGUGg -3' miRNA: 3'- gACGACGCa-------------CGUGCUGCGGCCCaCGC- -5' |
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| 11864 | 5' | -60.7 | NC_003266.2 | + | 15928 | 0.74 | 0.088836 |
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Target: 5'- --aCUGgGUGCGCGACGCCGccaccGGUGUGc -3' miRNA: 3'- gacGACgCACGUGCUGCGGC-----CCACGC- -5' |
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| 11864 | 5' | -60.7 | NC_003266.2 | + | 22379 | 1.07 | 0.000227 |
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Target: 5'- gCUGCUGCGUGCACGACGCCGGGUGCGc -3' miRNA: 3'- -GACGACGCACGUGCUGCGGCCCACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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