Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11866 | 3' | -55.9 | NC_003266.2 | + | 8447 | 0.66 | 0.58085 |
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Target: 5'- cGGAGG-CAGUggUGGcaggggcacgucGGCGCCGCgCGcGGGc -3' miRNA: 3'- -CCUUCaGUCA--AUC------------UCGCGGCG-GC-CCU- -5' |
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| 11866 | 3' | -55.9 | NC_003266.2 | + | 18086 | 0.66 | 0.58085 |
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Target: 5'- uGGGAGUgCAGagugUGaAGCGCCGCCGc-- -3' miRNA: 3'- -CCUUCA-GUCa---AUcUCGCGGCGGCccu -5' |
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| 11866 | 3' | -55.9 | NC_003266.2 | + | 31591 | 0.66 | 0.569716 |
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Target: 5'- uGGAGGa-AGUUuGcAGC-CCGCCGGGGc -3' miRNA: 3'- -CCUUCagUCAAuC-UCGcGGCGGCCCU- -5' |
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| 11866 | 3' | -55.9 | NC_003266.2 | + | 25422 | 0.66 | 0.563061 |
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Target: 5'- aGGAAG-CAGcaagaagcugaaagUGGAGCuGCCGcCCGuGGAg -3' miRNA: 3'- -CCUUCaGUCa-------------AUCUCG-CGGC-GGC-CCU- -5' |
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| 11866 | 3' | -55.9 | NC_003266.2 | + | 21903 | 0.67 | 0.483212 |
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Target: 5'- ---cGUCAGUUgcAGGGCGCCcagcagGUCGGGc -3' miRNA: 3'- ccuuCAGUCAA--UCUCGCGG------CGGCCCu -5' |
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| 11866 | 3' | -55.9 | NC_003266.2 | + | 16843 | 0.7 | 0.324488 |
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Target: 5'- cGggGUUuuc-GGGGCGCCGCCGGc- -3' miRNA: 3'- cCuuCAGucaaUCUCGCGGCGGCCcu -5' |
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| 11866 | 3' | -55.9 | NC_003266.2 | + | 27678 | 0.74 | 0.181296 |
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Target: 5'- uGGuuGUUAGUgaGGAGCGgCGCUGGGGa -3' miRNA: 3'- -CCuuCAGUCAa-UCUCGCgGCGGCCCU- -5' |
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| 11866 | 3' | -55.9 | NC_003266.2 | + | 23310 | 1.11 | 0.000354 |
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Target: 5'- aGGAAGUCAGUUAGAGCGCCGCCGGGAg -3' miRNA: 3'- -CCUUCAGUCAAUCUCGCGGCGGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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