Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11867 | 5' | -54.9 | NC_003266.2 | + | 32369 | 0.66 | 0.60539 |
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Target: 5'- uGCCA--GCCAUUAUG-GCUccUGUGCUGu -3' miRNA: 3'- -UGGUgcCGGUAAUACuCGA--ACGCGGC- -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 16900 | 0.66 | 0.582534 |
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Target: 5'- uACCACGGCUAUacc-GGCUccCGCCGc -3' miRNA: 3'- -UGGUGCCGGUAauacUCGAacGCGGC- -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 17297 | 0.66 | 0.571168 |
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Target: 5'- cGCCGCGGCgAUgAUGGGgaUcaGCGCgGg -3' miRNA: 3'- -UGGUGCCGgUAaUACUCgaA--CGCGgC- -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 18056 | 0.66 | 0.566637 |
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Target: 5'- gGCCACGGCCAccggcggcagGGGCUa--GCCa -3' miRNA: 3'- -UGGUGCCGGUaaua------CUCGAacgCGGc -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 10099 | 0.67 | 0.493598 |
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Target: 5'- --aGCGGCCAUcgcucgGUG-GCggggGCGCCGg -3' miRNA: 3'- uggUGCCGGUAa-----UACuCGaa--CGCGGC- -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 11232 | 0.68 | 0.472297 |
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Target: 5'- gGCCGCGGCCAc-GUGcGCgcGCGCa- -3' miRNA: 3'- -UGGUGCCGGUaaUACuCGaaCGCGgc -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 3708 | 0.7 | 0.346829 |
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Target: 5'- cCCAUGGCCAUUccGcGCacgGCGCUGg -3' miRNA: 3'- uGGUGCCGGUAAuaCuCGaa-CGCGGC- -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 13034 | 0.71 | 0.297518 |
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Target: 5'- uGCgCGCGGUCAUgucGAGCgcgGCGCUGg -3' miRNA: 3'- -UG-GUGCCGGUAauaCUCGaa-CGCGGC- -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 8799 | 0.72 | 0.282335 |
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Target: 5'- cUCGCGGCCAccgugGAGC--GCGCCGg -3' miRNA: 3'- uGGUGCCGGUaaua-CUCGaaCGCGGC- -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 10871 | 0.79 | 0.090735 |
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Target: 5'- cGCCGCGGCCGccGUGAGCg-GgGCCGg -3' miRNA: 3'- -UGGUGCCGGUaaUACUCGaaCgCGGC- -5' |
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| 11867 | 5' | -54.9 | NC_003266.2 | + | 24368 | 1.08 | 0.000663 |
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Target: 5'- gACCACGGCCAUUAUGAGCUUGCGCCGc -3' miRNA: 3'- -UGGUGCCGGUAAUACUCGAACGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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