miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1187 5' -50.6 NC_001132.2 + 29663 0.66 0.992581
Target:  5'- -aGCGGCGGACGAaccuccauUGGUCCGa--- -3'
miRNA:   3'- uaUGCCGCUUGCUcu------GCUAGGCaaaa -5'
1187 5' -50.6 NC_001132.2 + 34930 0.68 0.979555
Target:  5'- uGUACGGCGAuagguCGgcAGAUGAUCCa---- -3'
miRNA:   3'- -UAUGCCGCUu----GC--UCUGCUAGGcaaaa -5'
1187 5' -50.6 NC_001132.2 + 33427 0.68 0.977155
Target:  5'- cGUACGGCaGAACGGGAC---CCGUg-- -3'
miRNA:   3'- -UAUGCCG-CUUGCUCUGcuaGGCAaaa -5'
1187 5' -50.6 NC_001132.2 + 10647 0.68 0.977155
Target:  5'- -cACGGCGAuCGuGGCGAacaaaCCGUUUa -3'
miRNA:   3'- uaUGCCGCUuGCuCUGCUa----GGCAAAa -5'
1187 5' -50.6 NC_001132.2 + 9160 0.69 0.968718
Target:  5'- cUACGaagauCGAACGAGGCGGUgCCGUg-- -3'
miRNA:   3'- uAUGCc----GCUUGCUCUGCUA-GGCAaaa -5'
1187 5' -50.6 NC_001132.2 + 48694 0.69 0.95431
Target:  5'- -cGCGGUGAguuuauACGAGGCcuuGAUCUGUUUa -3'
miRNA:   3'- uaUGCCGCU------UGCUCUG---CUAGGCAAAa -5'
1187 5' -50.6 NC_001132.2 + 156727 1.04 0.018209
Target:  5'- gAUACGGCGAACGAGACGAUCCGUUUUg -3'
miRNA:   3'- -UAUGCCGCUUGCUCUGCUAGGCAAAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.