Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11871 | 5' | -54 | NC_003266.2 | + | 5408 | 0.66 | 0.67008 |
Target: 5'- cGGAcucGGGGgcguaggCgUCCGCGCCGCa -3' miRNA: 3'- -UCU---UCUCaaaaga-GgAGGCGCGGCGc -5' |
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11871 | 5' | -54 | NC_003266.2 | + | 31067 | 0.66 | 0.649535 |
Target: 5'- uGGgcGAGacg-CUgCUCCGaGCCGCGg -3' miRNA: 3'- -UCuuCUCaaaaGAgGAGGCgCGGCGC- -5' |
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11871 | 5' | -54 | NC_003266.2 | + | 16989 | 0.67 | 0.592377 |
Target: 5'- cGGAGAGUgua--CCgCCGCGgCCGCGc -3' miRNA: 3'- uCUUCUCAaaagaGGaGGCGC-GGCGC- -5' |
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11871 | 5' | -54 | NC_003266.2 | + | 23268 | 0.68 | 0.53389 |
Target: 5'- cGGAAGGGg---CUCUgccagccacccgCCGgGCCGCGa -3' miRNA: 3'- -UCUUCUCaaaaGAGGa-----------GGCgCGGCGC- -5' |
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11871 | 5' | -54 | NC_003266.2 | + | 17865 | 0.69 | 0.503234 |
Target: 5'- --cAGcAGcg-UCUCCUCCGCGUCGgGa -3' miRNA: 3'- ucuUC-UCaaaAGAGGAGGCGCGGCgC- -5' |
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11871 | 5' | -54 | NC_003266.2 | + | 7000 | 0.69 | 0.471269 |
Target: 5'- cGAAGAGUUccUUCUCaaCCGCgaGCUGUGg -3' miRNA: 3'- uCUUCUCAA--AAGAGgaGGCG--CGGCGC- -5' |
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11871 | 5' | -54 | NC_003266.2 | + | 527 | 1.1 | 0.000699 |
Target: 5'- gAGAAGAGUUUUCUCCUCCGCGCCGCGa -3' miRNA: 3'- -UCUUCUCAAAAGAGGAGGCGCGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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