Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11874 | 5' | -58.3 | NC_003266.2 | + | 16726 | 0.66 | 0.423887 |
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Target: 5'- aCGgUCUGc-ACGccAAGGCCCGGGgCCAc -3' miRNA: 3'- -GUgAGACucUGC--UUCCGGGCCCaGGU- -5' |
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| 11874 | 5' | -58.3 | NC_003266.2 | + | 12096 | 0.66 | 0.414342 |
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Target: 5'- aGCgccaUGAuGCGuugcauGGCCUGGGUCCAa -3' miRNA: 3'- gUGag--ACUcUGCuu----CCGGGCCCAGGU- -5' |
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| 11874 | 5' | -58.3 | NC_003266.2 | + | 17779 | 0.69 | 0.288132 |
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Target: 5'- cCACg--GAGccgGCGggGGgaaCCGGGUCCAg -3' miRNA: 3'- -GUGagaCUC---UGCuuCCg--GGCCCAGGU- -5' |
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| 11874 | 5' | -58.3 | NC_003266.2 | + | 8561 | 0.7 | 0.253348 |
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Target: 5'- -cCUCUGGG-UGAAGGCCaCGGGaCCc -3' miRNA: 3'- guGAGACUCuGCUUCCGG-GCCCaGGu -5' |
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| 11874 | 5' | -58.3 | NC_003266.2 | + | 10107 | 0.72 | 0.188977 |
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Target: 5'- uCGCUCgGuGGCGggGGCgCCGGGcgCUAg -3' miRNA: 3'- -GUGAGaCuCUGCuuCCG-GGCCCa-GGU- -5' |
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| 11874 | 5' | -58.3 | NC_003266.2 | + | 6657 | 0.73 | 0.147461 |
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Target: 5'- aACUCgugcgagGGGGCGAGGaGCCCGGcUCCGa -3' miRNA: 3'- gUGAGa------CUCUGCUUC-CGGGCCcAGGU- -5' |
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| 11874 | 5' | -58.3 | NC_003266.2 | + | 5661 | 1.08 | 0.000333 |
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Target: 5'- cCACUCUGAGACGAAGGCCCGGGUCCAa -3' miRNA: 3'- -GUGAGACUCUGCUUCCGGGCCCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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