Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11876 | 5' | -58.6 | NC_003266.2 | + | 10923 | 0.68 | 0.354369 |
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Target: 5'- -cUCACGGcGGCCGCGGcGGUag-UGGc -3' miRNA: 3'- gaGGUGCCaCCGGCGCU-CCAgcaACC- -5' |
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| 11876 | 5' | -58.6 | NC_003266.2 | + | 14110 | 0.68 | 0.337831 |
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Target: 5'- -aCCACGGUGGUCagaaaguuGCuGuGGUCGUucUGGu -3' miRNA: 3'- gaGGUGCCACCGG--------CG-CuCCAGCA--ACC- -5' |
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| 11876 | 5' | -58.6 | NC_003266.2 | + | 15691 | 0.68 | 0.321862 |
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Target: 5'- gUCCACGGUGGagacgggCGCGGcGG-CGggGGu -3' miRNA: 3'- gAGGUGCCACCg------GCGCU-CCaGCaaCC- -5' |
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| 11876 | 5' | -58.6 | NC_003266.2 | + | 9210 | 0.69 | 0.277375 |
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Target: 5'- cCUCCucagGCGGUGGUgGCGGGGgaGggGGc -3' miRNA: 3'- -GAGG----UGCCACCGgCGCUCCagCaaCC- -5' |
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| 11876 | 5' | -58.6 | NC_003266.2 | + | 9372 | 0.76 | 0.102342 |
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Target: 5'- uCUCCA-GGUGGcCCGgGGGGUcccCGUUGGg -3' miRNA: 3'- -GAGGUgCCACC-GGCgCUCCA---GCAACC- -5' |
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| 11876 | 5' | -58.6 | NC_003266.2 | + | 8746 | 1.1 | 0.000264 |
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Target: 5'- gCUCCACGGUGGCCGCGAGGUCGUUGGa -3' miRNA: 3'- -GAGGUGCCACCGGCGCUCCAGCAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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