Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11877 | 3' | -56.2 | NC_003266.2 | + | 18667 | 0.66 | 0.507379 |
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Target: 5'- aCUGgcaGGugUugGGCGCucCCUUGGGAg- -3' miRNA: 3'- -GACa--UUugGugCCGCG--GGAGCCCUag -5' |
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| 11877 | 3' | -56.2 | NC_003266.2 | + | 12122 | 0.67 | 0.49672 |
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Target: 5'- cCUGgaGGCCGUGGUGCCCUCGcGcUCc -3' miRNA: 3'- -GACauUUGGUGCCGCGGGAGCcCuAG- -5' |
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| 11877 | 3' | -56.2 | NC_003266.2 | + | 22252 | 0.67 | 0.486163 |
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Target: 5'- cCUGcUGGGCCuuGGCuCCCUCGGuGAa- -3' miRNA: 3'- -GAC-AUUUGGugCCGcGGGAGCC-CUag -5' |
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| 11877 | 3' | -56.2 | NC_003266.2 | + | 16497 | 0.68 | 0.415552 |
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Target: 5'- gCUGccGAgCGCGGCGCCgCgUCGGGGc- -3' miRNA: 3'- -GACauUUgGUGCCGCGG-G-AGCCCUag -5' |
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| 11877 | 3' | -56.2 | NC_003266.2 | + | 5676 | 0.68 | 0.405984 |
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Target: 5'- ---aGGACCGCGGCGUCCcacUCGaGGAc- -3' miRNA: 3'- gacaUUUGGUGCCGCGGG---AGC-CCUag -5' |
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| 11877 | 3' | -56.2 | NC_003266.2 | + | 22568 | 0.68 | 0.40031 |
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Target: 5'- gCUGUAccgaagucgagggcaAGCUGCGGUGCCUgcaCGGGAc- -3' miRNA: 3'- -GACAU---------------UUGGUGCCGCGGGa--GCCCUag -5' |
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| 11877 | 3' | -56.2 | NC_003266.2 | + | 24288 | 0.69 | 0.375413 |
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Target: 5'- -cGUcgcuGACCACgGGCuugcccuccucggaGCUCUCGGGGUCu -3' miRNA: 3'- gaCAu---UUGGUG-CCG--------------CGGGAGCCCUAG- -5' |
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| 11877 | 3' | -56.2 | NC_003266.2 | + | 27670 | 0.71 | 0.29041 |
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Target: 5'- -aGUGAGgaGCGGCGCUggggaagaggugguaUUCGGGAUCa -3' miRNA: 3'- gaCAUUUggUGCCGCGG---------------GAGCCCUAG- -5' |
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| 11877 | 3' | -56.2 | NC_003266.2 | + | 8828 | 1.1 | 0.000383 |
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Target: 5'- gCUGUAAACCACGGCGCCCUCGGGAUCg -3' miRNA: 3'- -GACAUUUGGUGCCGCGGGAGCCCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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