Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11877 | 5' | -57.9 | NC_003266.2 | + | 23839 | 0.66 | 0.409999 |
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Target: 5'- -aGACCggguagaAgUUGaGGCGuGGGUUGGGCUCg -3' miRNA: 3'- uaCUGG-------UgGAC-CCGC-UCCAACUCGAG- -5' |
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| 11877 | 5' | -57.9 | NC_003266.2 | + | 7207 | 0.66 | 0.382725 |
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Target: 5'- -cGugCGCUUcuuguaGGCGGGGUUGGGCa- -3' miRNA: 3'- uaCugGUGGAc-----CCGCUCCAACUCGag -5' |
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| 11877 | 5' | -57.9 | NC_003266.2 | + | 23793 | 0.66 | 0.379974 |
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Target: 5'- -gGGCUggcGCUugggGGGCacgcgcggcgagaaGAGGUUGAGCUCg -3' miRNA: 3'- uaCUGG---UGGa---CCCG--------------CUCCAACUCGAG- -5' |
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| 11877 | 5' | -57.9 | NC_003266.2 | + | 18030 | 0.67 | 0.364632 |
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Target: 5'- --aGCCACUguggagGGGCGGGGagUGGGCg- -3' miRNA: 3'- uacUGGUGGa-----CCCGCUCCa-ACUCGag -5' |
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| 11877 | 5' | -57.9 | NC_003266.2 | + | 16368 | 0.67 | 0.35581 |
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Target: 5'- -cGAgCGCCUGGGCGAGuUUGcuuacGGCa- -3' miRNA: 3'- uaCUgGUGGACCCGCUCcAAC-----UCGag -5' |
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| 11877 | 5' | -57.9 | NC_003266.2 | + | 23072 | 0.67 | 0.330254 |
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Target: 5'- gGUGGuuGuuCCUGaGGCGAGGggGAGCg- -3' miRNA: 3'- -UACUggU--GGAC-CCGCUCCaaCUCGag -5' |
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| 11877 | 5' | -57.9 | NC_003266.2 | + | 13394 | 0.7 | 0.22296 |
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Target: 5'- --cGCUugCUGGGCGAGGagGAGUa- -3' miRNA: 3'- uacUGGugGACCCGCUCCaaCUCGag -5' |
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| 11877 | 5' | -57.9 | NC_003266.2 | + | 3777 | 0.71 | 0.194407 |
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Target: 5'- uUGACCcagcGCCUGGGCGAgcugacccagcaGGU--GGCUCa -3' miRNA: 3'- uACUGG----UGGACCCGCU------------CCAacUCGAG- -5' |
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| 11877 | 5' | -57.9 | NC_003266.2 | + | 8864 | 1.06 | 0.000385 |
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Target: 5'- cAUGACCACCUGGGCGAGGUUGAGCUCc -3' miRNA: 3'- -UACUGGUGGACCCGCUCCAACUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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