Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11880 | 3' | -54 | NC_003266.2 | + | 13392 | 0.66 | 0.666621 |
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Target: 5'- cGCGCuUGCUGGgcgaggaggaGUAcuuaaauaacUCGCUGuugaGGCCCGAg -3' miRNA: 3'- -CGCG-ACGACU----------UAU----------AGUGGC----UCGGGCU- -5' |
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| 11880 | 3' | -54 | NC_003266.2 | + | 26381 | 0.66 | 0.666621 |
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Target: 5'- cCGCa--UGAAUuggcucagCGCCGGGCCCGGg -3' miRNA: 3'- cGCGacgACUUAua------GUGGCUCGGGCU- -5' |
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| 11880 | 3' | -54 | NC_003266.2 | + | 3745 | 0.69 | 0.48672 |
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Target: 5'- aGCuGCUGCUGuugAUgGCCcAGCUCGAg -3' miRNA: 3'- -CG-CGACGACuuaUAgUGGcUCGGGCU- -5' |
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| 11880 | 3' | -54 | NC_003266.2 | + | 23656 | 0.7 | 0.414995 |
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Target: 5'- cGCGCUGCuUGAccg-CACCGAgguGCCCc- -3' miRNA: 3'- -CGCGACG-ACUuauaGUGGCU---CGGGcu -5' |
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| 11880 | 3' | -54 | NC_003266.2 | + | 19238 | 0.7 | 0.405285 |
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Target: 5'- aGUGCUGUUGuAGUA-CA-UGAGCCCGAu -3' miRNA: 3'- -CGCGACGAC-UUAUaGUgGCUCGGGCU- -5' |
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| 11880 | 3' | -54 | NC_003266.2 | + | 22382 | 0.7 | 0.395719 |
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Target: 5'- cGCGCUGCUGcGUGcacgaCGCCGGGUgCGc -3' miRNA: 3'- -CGCGACGACuUAUa----GUGGCUCGgGCu -5' |
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| 11880 | 3' | -54 | NC_003266.2 | + | 11422 | 1.11 | 0.000528 |
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Target: 5'- gGCGCUGCUGAAUAUCACCGAGCCCGAg -3' miRNA: 3'- -CGCGACGACUUAUAGUGGCUCGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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