Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11881 | 3' | -53.3 | NC_003266.2 | + | 6525 | 0.66 | 0.735237 |
Target: 5'- gCCGUgcgcgaaUGGCA--AGCUUCUCuGGACGAu -3' miRNA: 3'- -GGCAg------GCCGUaaUUGAGGAG-CCUGCU- -5' |
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11881 | 3' | -53.3 | NC_003266.2 | + | 27153 | 0.67 | 0.655226 |
Target: 5'- uCCGUgggCCgGGCccugaggguGCUCCUCGGACa- -3' miRNA: 3'- -GGCA---GG-CCGuaau-----UGAGGAGCCUGcu -5' |
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11881 | 3' | -53.3 | NC_003266.2 | + | 6367 | 0.68 | 0.634869 |
Target: 5'- -gGUCCaGCAUaAGCUCgUCGGGgGGg -3' miRNA: 3'- ggCAGGcCGUAaUUGAGgAGCCUgCU- -5' |
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11881 | 3' | -53.3 | NC_003266.2 | + | 12002 | 1.11 | 0.000851 |
Target: 5'- gCCGUCCGGCAUUAACUCCUCGGACGAu -3' miRNA: 3'- -GGCAGGCCGUAAUUGAGGAGCCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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