Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11884 | 3' | -56.1 | NC_003266.2 | + | 24370 | 0.66 | 0.519858 |
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Target: 5'- uUCGCCGACGCagaaAUUCUgcgcaaggucgaggaGAACCUgCACu -3' miRNA: 3'- -AGUGGUUGCGg---UAGGA---------------CUUGGGgGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 25032 | 0.67 | 0.479261 |
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Target: 5'- cUCugC-ACGCCGcaccgcucccuggCCUGcAACCCCCAg -3' miRNA: 3'- -AGugGuUGCGGUa------------GGAC-UUGGGGGUg -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 26353 | 0.71 | 0.263216 |
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Target: 5'- cCGCCGGCGCCGcCCaGGACUacuccaCCCGCa -3' miRNA: 3'- aGUGGUUGCGGUaGGaCUUGG------GGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 27170 | 0.67 | 0.472991 |
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Target: 5'- -aGCCAGCGCCcgAUCCUGGuCCagcgCCAa -3' miRNA: 3'- agUGGUUGCGG--UAGGACUuGGg---GGUg -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 27653 | 0.66 | 0.546184 |
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Target: 5'- cCACCAACGCUA-CCgucgagaccucguUGAaucuaauACCaCCCACa -3' miRNA: 3'- aGUGGUUGCGGUaGG-------------ACU-------UGG-GGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 30450 | 0.67 | 0.442287 |
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Target: 5'- aUCGCCuaccuGCGCCA-CC---ACCCCCAg -3' miRNA: 3'- -AGUGGu----UGCGGUaGGacuUGGGGGUg -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 31158 | 0.68 | 0.403166 |
|
Target: 5'- cCACCGAccauCGCCucUCCUacGAGCUCCUGCa -3' miRNA: 3'- aGUGGUU----GCGGu-AGGA--CUUGGGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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