Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11884 | 3' | -56.1 | NC_003266.2 | + | 12101 | 0.74 | 0.160789 |
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Target: 5'- -gGCCAACcggcucucgGCCAUCCUGGaggccgugguGCCCUCGCg -3' miRNA: 3'- agUGGUUG---------CGGUAGGACU----------UGGGGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 11952 | 0.68 | 0.393734 |
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Target: 5'- gCAgCAACaGCCAccUCCUGA-UCCCCAa -3' miRNA: 3'- aGUgGUUG-CGGU--AGGACUuGGGGGUg -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 9670 | 0.68 | 0.393733 |
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Target: 5'- cCACCAucCGCCGUCUcaGAACCgCCUAUu -3' miRNA: 3'- aGUGGUu-GCGGUAGGa-CUUGG-GGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 8496 | 0.67 | 0.452398 |
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Target: 5'- cCACCAcUGCCuccggGUCCcGAgccGCCCCUACc -3' miRNA: 3'- aGUGGUuGCGG-----UAGGaCU---UGGGGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 8337 | 0.69 | 0.360984 |
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Target: 5'- cCACCAacgGCGCgGUggagaucaaguaccaUCUGGACCuCCCGCg -3' miRNA: 3'- aGUGGU---UGCGgUA---------------GGACUUGG-GGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 3643 | 0.68 | 0.422455 |
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Target: 5'- cCGCCAGCGCCGUgcgcggaauggCCaUGGGCgCCgGCu -3' miRNA: 3'- aGUGGUUGCGGUA-----------GG-ACUUGgGGgUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 662 | 0.73 | 0.195513 |
|
Target: 5'- -gGgCGACGaCCAUCCgGAAcCCCCCACc -3' miRNA: 3'- agUgGUUGC-GGUAGGaCUU-GGGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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