Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11884 | 3' | -56.1 | NC_003266.2 | + | 26353 | 0.71 | 0.263216 |
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Target: 5'- cCGCCGGCGCCGcCCaGGACUacuccaCCCGCa -3' miRNA: 3'- aGUGGUUGCGGUaGGaCUUGG------GGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 11952 | 0.68 | 0.393734 |
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Target: 5'- gCAgCAACaGCCAccUCCUGA-UCCCCAa -3' miRNA: 3'- aGUgGUUG-CGGU--AGGACUuGGGGGUg -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 31158 | 0.68 | 0.403166 |
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Target: 5'- cCACCGAccauCGCCucUCCUacGAGCUCCUGCa -3' miRNA: 3'- aGUGGUU----GCGGu-AGGA--CUUGGGGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 3643 | 0.68 | 0.422455 |
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Target: 5'- cCGCCAGCGCCGUgcgcggaauggCCaUGGGCgCCgGCu -3' miRNA: 3'- aGUGGUUGCGGUA-----------GG-ACUUGgGGgUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 13679 | 0.68 | 0.432304 |
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Target: 5'- -aACCcguuCGCUcaCCUGcGCCCCCGCg -3' miRNA: 3'- agUGGuu--GCGGuaGGACuUGGGGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 15605 | 0.68 | 0.432304 |
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Target: 5'- -gGCCGACGCUcgCaacuacACCCCCGCc -3' miRNA: 3'- agUGGUUGCGGuaGgacu--UGGGGGUG- -5' |
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| 11884 | 3' | -56.1 | NC_003266.2 | + | 13091 | 1.1 | 0.00039 |
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Target: 5'- uUCACCAACGCCAUCCUGAACCCCCACu -3' miRNA: 3'- -AGUGGUUGCGGUAGGACUUGGGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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